Cited 12 times in
Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach
DC Field | Value | Language |
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dc.contributor.author | 박중원 | - |
dc.contributor.author | 이재현 | - |
dc.contributor.author | 정경용 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2016-02-04T11:14:55Z | - |
dc.date.available | 2016-02-04T11:14:55Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2092-7355 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139998 | - |
dc.description.abstract | PURPOSE: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. METHODS: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. RESULTS: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. CONCLUSIONS: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 283~289 | - |
dc.relation.isPartOf | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute of Allergy (알레르기연구소) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Mina Son | - |
dc.contributor.googleauthor | Jae-Hyun Lee | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.identifier.doi | 10.4168/aair.2015.7.3.283 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A03086 | - |
dc.contributor.localId | A03572 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J00064 | - |
dc.identifier.eissn | 2092-7363 | - |
dc.identifier.pmid | 25749759 | - |
dc.subject.keyword | Allergens | - |
dc.subject.keyword | German cockroach | - |
dc.subject.keyword | chymotrypsin | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Lee, Jae Hyun | - |
dc.contributor.alternativeName | Jeong, Kyoung Yong | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hyun | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 7 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 283 | - |
dc.citation.endPage | 289 | - |
dc.identifier.bibliographicCitation | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH, Vol.7(3) : 283-289, 2015 | - |
dc.identifier.rimsid | 49019 | - |
dc.type.rims | ART | - |
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