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Conserved role of Sonic Hedgehog in tonotopic organization of the avian basilar papilla and mammalian cochlea

Authors
 Eun Jin Son  ;  Ji-Hyun Ma  ;  Harinarayana Ankamreddy  ;  Jeong-Oh Shin  ;  Jae Young Choi  ;  Doris K. Wu  ;  Jinwoong Bok 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.112(12) : 3746-3751, 2015 
Journal Title
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 
ISSN
 0027-8424 
Issue Date
2015
MeSH
Animals ; Chickens ; Cochlea/metabolism* ; Cochlea/physiology ; Hair Cells, Auditory/metabolism ; Hearing/physiology* ; Hedgehog Proteins/metabolism* ; Mechanotransduction, Cellular* ; Mice ; Notochord/metabolism ; Organ of Corti/metabolism ; Organ of Corti/physiology ; Phenotype ; Signal Transduction ; Species Specificity
Keywords
A2m ; Efnb2 ; Inhba ; frequency discrimination ; regional identity
Abstract
Sound frequency discrimination begins at the organ of Corti in mammals and the basilar papilla in birds. Both of these hearing organs are tonotopically organized such that sensory hair cells at the basal (proximal) end respond to high frequency sound, whereas their counterparts at the apex (distal) respond to low frequencies. Sonic hedgehog (Shh) secreted by the developing notochord and floor plate is required for cochlear formation in both species. In mice, the apical region of the developing cochlea, closer to the ventral midline source of Shh, requires higher levels of Shh signaling than the basal cochlea farther away from the midline. Here, gain-of-function experiments using Shh-soaked beads in ovo or a mouse model expressing constitutively activated Smoothened (transducer of Shh signaling) show up-regulation of apical genes in the basal cochlea, even though these regionally expressed genes are not necessarily conserved between the two species. In chicken, these altered gene expression patterns precede morphological and physiological changes in sensory hair cells that are typically associated with tonotopy such as the total number of stereocilia per hair cell and gene expression of an inward rectifier potassium channel, IRK1, which is a bona fide feature of apical hair cells in the basilar papilla. Furthermore, our results suggest that this conserved role of Shh in establishing cochlear tonotopy is initiated early in development by Shh emanating from the notochord and floor plate.
Files in This Item:
T201500962.pdf Download
DOI
10.1073/pnas.1417856112
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Ma, Ji Hyun(마지현) ORCID logo https://orcid.org/0000-0002-7215-9040
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
Son, Eun Jin(손은진)
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Ankamreddy, Harinarayana(안캄레디하리) ORCID logo https://orcid.org/0000-0002-2859-087X
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139827
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