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Apoptotic effect of pheophorbide a-mediated photodynamic therapy on DMBA/TPA-induced mouse papillomas

 Xianglan Zhang  ;  Eun Joo Choi  ;  Zhenlong Zheng  ;  Lianhua Zhu  ;  Sung Bin Cho  ;  Ki-Yoel Kim  ;  Jin Kim  ;  In-Ho Cha 
 LASERS IN MEDICAL SCIENCE, Vol.30(1) : 51-57, 2015 
Journal Title
Issue Date
9,10-Dimethyl-1,2-benzanthracene ; Animals ; Apoptosis/drug effects* ; Carcinogenesis/drug effects ; Carcinogenesis/pathology ; Caspase 3/metabolism ; Chlorophyll/analogs & derivatives* ; Chlorophyll/pharmacology ; Chlorophyll/therapeutic use ; Female ; Immunohistochemistry ; In Situ Nick-End Labeling ; Mice, Hairless ; Papilloma/drug therapy* ; Papilloma/metabolism ; Papilloma/pathology ; Photochemotherapy* ; Photosensitizing Agents/pharmacology ; Photosensitizing Agents/therapeutic use ; Proliferating Cell Nuclear Antigen/metabolism ; Tetradecanoylphorbol Acetate
Photodynamic therapy ; Pheophorbide a ; Papilloma ; Mousemodel
Pheophorbide a (Pa) is a chlorine-based photosensitizer, and Pa-mediated photodynamic therapy (PDT) reportedly exhibits antitumor activity against various malignancies. The aim of our study was to investigate the therapeutic effect of Pa-mediated PDT on 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorobol-13-acetate (TPA)-induced mouse papillomas. Thirty mice received a topical application of DMBA/TPA on their backs to induce mouse papillomas. One week after two sessions of Pa-mediated PDT, immunohistochemical stains and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed to evaluate the apoptotic effects thereof on the papillomas. Among 63 mouse papillomas treated with Pa-mediated PDT, 17.5% of the lesions were completely removed 1 week after the first treatment, while 31.7% disappeared 1 week after the second treatment. Statistical analyses revealed significant differences in therapeutic outcomes for the Pa-mediated PDT group in comparison to a solvent-PDT group and a Pa group. Additionally, a marked downregulation of proliferating cell nuclear antigen expression, as well as upregulation of cleaved caspase 3 and cleaved poly(ADP-ribose) polymerase expression, was noted in the Pa-PDT group, compared to the solvent-PDT group and Pa group. TUNEL assay revealed higher apoptotic cell counts in the Pa-PDT group, although the difference was not statistically significant. Our data demonstrated that Pa-mediated PDT is effective in treating DMBA/TPA-induced mouse papillomas.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Zhang, Xiang Lan(장향란)
Zheng, Zhen Long(정진룡)
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
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