0 414

Cited 3 times in

Apoptotic effect of pheophorbide a-mediated photodynamic therapy on DMBA/TPA-induced mouse papillomas

DC FieldValueLanguage
dc.contributor.author김진-
dc.contributor.author장향란-
dc.contributor.author정진룡-
dc.contributor.author차인호-
dc.date.accessioned2016-02-04T10:54:59Z-
dc.date.available2016-02-04T10:54:59Z-
dc.date.issued2015-
dc.identifier.issn0268-8921-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139261-
dc.description.abstractPheophorbide a (Pa) is a chlorine-based photosensitizer, and Pa-mediated photodynamic therapy (PDT) reportedly exhibits antitumor activity against various malignancies. The aim of our study was to investigate the therapeutic effect of Pa-mediated PDT on 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorobol-13-acetate (TPA)-induced mouse papillomas. Thirty mice received a topical application of DMBA/TPA on their backs to induce mouse papillomas. One week after two sessions of Pa-mediated PDT, immunohistochemical stains and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed to evaluate the apoptotic effects thereof on the papillomas. Among 63 mouse papillomas treated with Pa-mediated PDT, 17.5% of the lesions were completely removed 1 week after the first treatment, while 31.7% disappeared 1 week after the second treatment. Statistical analyses revealed significant differences in therapeutic outcomes for the Pa-mediated PDT group in comparison to a solvent-PDT group and a Pa group. Additionally, a marked downregulation of proliferating cell nuclear antigen expression, as well as upregulation of cleaved caspase 3 and cleaved poly(ADP-ribose) polymerase expression, was noted in the Pa-PDT group, compared to the solvent-PDT group and Pa group. TUNEL assay revealed higher apoptotic cell counts in the Pa-PDT group, although the difference was not statistically significant. Our data demonstrated that Pa-mediated PDT is effective in treating DMBA/TPA-induced mouse papillomas.-
dc.description.statementOfResponsibilityopen-
dc.format.extent51~57-
dc.relation.isPartOfLASERS IN MEDICAL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH9,10-Dimethyl-1,2-benzanthracene-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHCarcinogenesis/drug effects-
dc.subject.MESHCarcinogenesis/pathology-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHChlorophyll/analogs & derivatives*-
dc.subject.MESHChlorophyll/pharmacology-
dc.subject.MESHChlorophyll/therapeutic use-
dc.subject.MESHFemale-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHIn Situ Nick-End Labeling-
dc.subject.MESHMice, Hairless-
dc.subject.MESHPapilloma/drug therapy*-
dc.subject.MESHPapilloma/metabolism-
dc.subject.MESHPapilloma/pathology-
dc.subject.MESHPhotochemotherapy*-
dc.subject.MESHPhotosensitizing Agents/pharmacology-
dc.subject.MESHPhotosensitizing Agents/therapeutic use-
dc.subject.MESHProliferating Cell Nuclear Antigen/metabolism-
dc.subject.MESHTetradecanoylphorbol Acetate-
dc.titleApoptotic effect of pheophorbide a-mediated photodynamic therapy on DMBA/TPA-induced mouse papillomas-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorXianglan Zhang-
dc.contributor.googleauthorEun Joo Choi-
dc.contributor.googleauthorZhenlong Zheng-
dc.contributor.googleauthorLianhua Zhu-
dc.contributor.googleauthorSung Bin Cho-
dc.contributor.googleauthorKi-Yoel Kim-
dc.contributor.googleauthorJin Kim-
dc.contributor.googleauthorIn-Ho Cha-
dc.identifier.doi10.1007/s10103-014-1615-3-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03489-
dc.contributor.localIdA03741-
dc.contributor.localIdA04002-
dc.contributor.localIdA01009-
dc.relation.journalcodeJ02158-
dc.identifier.eissn1435-604X-
dc.identifier.pmid24950744-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs10103-014-1615-3-
dc.subject.keywordPhotodynamic therapy-
dc.subject.keywordPheophorbide a-
dc.subject.keywordPapilloma-
dc.subject.keywordMousemodel-
dc.contributor.alternativeNameKim, Jin-
dc.contributor.alternativeNameZhang, Xiang Lan-
dc.contributor.alternativeNameZheng, Zhen Long-
dc.contributor.alternativeNameCha, In Ho-
dc.contributor.affiliatedAuthorZhang, Xiang Lan-
dc.contributor.affiliatedAuthorZheng, Zhen Long-
dc.contributor.affiliatedAuthorCha, In Ho-
dc.contributor.affiliatedAuthorKim, Jin-
dc.rights.accessRightsnot free-
dc.citation.volume30-
dc.citation.number1-
dc.citation.startPage51-
dc.citation.endPage57-
dc.identifier.bibliographicCitationLASERS IN MEDICAL SCIENCE, Vol.30(1) : 51-57, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.