0 371

Cited 2 times in

Neuroprotective effect of 3-morpholinosydnonimine against Zn2+-induced PC12 cell death

Authors
 Jeong Mi An  ;  Seong Ah Moon  ;  Soo Young Hong  ;  Jeong Wan Kang  ;  Jeong Taeg Seo 
Citation
 EUROPEAN JOURNAL OF PHARMACOLOGY, Vol.748 : 37-44, 2015 
Journal Title
 EUROPEAN JOURNAL OF PHARMACOLOGY 
ISSN
 0014-2999 
Issue Date
2015
MeSH
Animals ; Cell Death/drug effects ; Enzyme Activation/drug effects ; Glutathione Disulfide/metabolism ; Glutathione Reductase/metabolism ; Humans ; Molsidomine/analogs & derivatives* ; Molsidomine/pharmacology ; Neurons/cytology ; Neurons/drug effects ; Neurons/metabolism ; Neuroprotective Agents/pharmacology* ; PC12 Cells ; Rats ; Zinc/adverse effects* ; Zinc/metabolism
Keywords
Glutathione reductase ; Neuroprotection ; PC12 cells ; SIN-1 ; Zn(2+)
Abstract
Excessive intracellular accumulation of zinc (Zn2+) is neurotoxic and contributes to a number of neuropathological conditions. Here, we investigated the protective effect of 3-morpholinosydnonimine (SIN-1) against Zn2+-induced neuronal cell death in differentiated PC12 cells. We found that Zn2+-induced PC12 cell death was reduced in a concentration-dependent manner by pretreatment with SIN-1. The intracellular accumulation of Zn2+ was not affected by pretreatment with SIN-1, indicating that SIN-1-induced neuroprotection was not attributable to reduced influx of Zn2+ into cells. SIN-1C, the stable decomposition product of SIN-1, failed to prevent Zn2+-induced cell death. Furthermore, the protective effect of SIN-1 against Zn2+-induced PC12 cell death was almost completely abolished by uric acid, a free radical scavenger, suggesting that reactive oxygen and nitrogen species generated by SIN-1 may contribute to the protective effect. SIN-1 prevented the inactivation of glutathione reductase (GR) and the increase in the ratio of oxidized glutathione/total glutathione (GSSG/total GSH) induced by Zn2+. Addition of membrane permeable GSH ethyl ester (GSH-EE) to PC12 cells prior to Zn2+ treatment significantly increased cell viability. We therefore conclude that SIN-1 may exert neuroprotective effect against Zn2+-induced cell death in differentiated PC12 cells by preventing inhibition of GR and increase in GSSG/total GSH ratio.
Full Text
http://www.sciencedirect.com/science/article/pii/S0014299914008619
DOI
10.1016/j.ejphar.2014.12.003
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kang, Jeong Wan(강정완) ORCID logo https://orcid.org/0000-0002-3945-8452
Moon, Seong Ah(문성아)
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
An, Jeong Mi(안정미)
Hong, Soo Young(홍수영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139234
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse