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The effect of ASK1 on vascular permeability and edema formation in cerebral ischemia

 JuhyunSong  ;  SoYeongCheon  ;  WonTaekLee  ;  KyungAhPark  ;  JongEunLee 
 BRAIN RESEARCH, Vol.1595 : 143-155, 2015 
Journal Title
Issue Date
Animals ; Aquaporin 1/metabolism ; Blood-Brain Barrier/drug effects ; Brain/drug effects ; Brain/metabolism ; Brain Edema/drug therapy ; Brain Edema/etiology* ; Brain Ischemia/complications* ; Brain Ischemia/pathology ; Capillary Permeability/drug effects ; Capillary Permeability/physiology* ; Cell Hypoxia/drug effects ; Cells, Cultured ; Disease Models, Animal ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Gene Expression Regulation/drug effects ; Gene Expression Regulation/physiology* ; Glucose/deficiency ; Male ; Mice ; Mice, Inbred C57BL ; RNA, Small Interfering/pharmacology ; Time Factors ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism*
Apoptosis signal-regulating kinase 1 (ASK1) ; Aquaporin-1 (AQP-1) ; Blood–brain barrier (BBB) ; Cerebral ischemia ; Edema ; Vascular endothelial growth factor (VEGF) ; Vascular permeability
Apoptosis signal-regulating kinase-1 (ASK1) is the mitogen-activated protein kinase kinase kinase (MAPKKK) and participates in the various central nervous system (CNS) signaling pathways. In cerebral ischemia, vascular permeability in the brain is an important issue because regulation failure of it results in edema formation and blood-brain barrier (BBB) disruption. To determine the role of ASK1 on vascular permeability and edema formation following cerebral ischemia, we first investigated ASK1-related gene expression using microarray analyses of ischemic brain tissue. We then measured protein levels of ASK1 and vascular endothelial growth factor (VEGF) in brain endothelial cells after hypoxia injury. We also examined protein expression of ASK1 and VEGF, edema formation, and morphological alteration through cresyl violet staining in ischemic brain tissue using ASK1-small interference RNA (ASK1-siRNA). Finally, immunohistochemistry was performed to examine VEGF and aquaporin-1 (AQP-1) expression in ischemic brain injury. Based on our findings, we propose that ASK1 is a regulating factor of vascular permeability and edema formation in cerebral ischemia
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1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Ah(박경아)
Song, Ju Hyun(송주현)
Lee, Won Taek(이원택) ORCID logo https://orcid.org/0000-0001-7348-9562
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
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