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Gene therapy using hepatocyte growth factor expressing adenovirus improves skin flap survival in a rat model

 Dong Kyun Rah  ;  In Sik Yun  ;  Chae Ok Yun  ;  Sae Bin Lee  ;  Won Jai Lee 
 Journal of Korean Medical Science, Vol.29(Suppl 3) : 228-236, 2014 
Journal Title
 Journal of Korean Medical Science 
Issue Date
Adenoviridae/genetics ; Animals ; Genetic Therapy/methods* ; Graft Survival/genetics ; Hepatocyte Growth Factor/biosynthesis ; Hepatocyte Growth Factor/genetics* ; Male ; Models, Animal ; Neovascularization, Physiologic/genetics* ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reconstructive Surgical Procedures ; Skin Transplantation/methods* ; Surgical Flaps/surgery*
Adenovirus ; Gene Therapy ; Hepatocyte Growth Factor ; Skin Flap
Hepatocyte growth factor (HGF) is a potent angiogenic factor that can stimulate the production of blood vessels in ischemic tissue. We investigated whether gene therapy using HGF-expressing adenovirus could enhance skin flap survival. Sprague-Dawley rats were randomly divided into three groups. Rats were subdermally injected with HGF-expressing adenovirus (HGF virus group), recombinant HGF (rhHGF group), or phosphate buffered saline (PBS group) 2 days before and immediately after 3 × 9 cm caudal flap elevation. The survival area of the skin flap, the ratio of blood flow, CD31-positive vessels and, VEGF expression were examined. Skin flap viability was significantly increased in the HGF virus group compared to the rhHGF and PBS groups (71.4% ± 5.9%, 63.8%± 6.4%, and 39.2% ± 13.0%, respectively) (P = 0.025). Furthermore, the blood flow ratio was significantly increased in the HGF virus group. In the HGF virus group, the number of CD31-positive vessels and vascular endothelial growth factor (VEGF) expression were significantly increased. Gene therapy using HGF-expressing adenovirus increase VEGF expression, the number of viable capillaries, and blood flow to the flap, thereby improving skin flap survival.
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1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Rah, Dong Kyun(나동균)
Yun, In Sik(윤인식) ORCID logo https://orcid.org/0000-0003-1103-7047
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
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