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Effect of endogenous bone marrow derived stem cells induced by AMD-3100 on expanded ischemic flap.

 Hii Sun Jeong  ;  Hye Kyung Lee  ;  Kwan Chul Tark  ;  Dae Hyun Lew  ;  Yoon Woo Koh  ;  Chul Hoon Kim  ;  In Suck Seo 
 JOURNAL OF KOREAN MEDICAL SCIENCE, Vol.29(Suppl 3) : 237-248, 2014 
Journal Title
Issue Date
Animals ; Anti-HIV Agents/pharmacology ; Bone Marrow Cells/cytology ; Chemokine CXCL12/biosynthesis ; Endothelial Progenitor Cells/cytology* ; Hematopoietic Stem Cells/cytology* ; Heterocyclic Compounds/pharmacology* ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Male ; Neovascularization, Physiologic ; Nitric Oxide Synthase Type III/metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, CXCR4/antagonists & inhibitors ; Surgical Flaps/blood supply* ; Surgical Flaps/surgery ; Tissue Expansion/methods* ; Vascular Endothelial Growth Factor A/biosynthesis ; Vascular Endothelial Growth Factor Receptor-2/biosynthesis ; Vascular Endothelial Growth Factor Receptor-2/metabolism
AMD-3100 ; CXCR4 Inhibitor ; Endothelial Progenitor Cell ; Hematopoietic Stem Cell ; Ischemic Flap ; Plerixafor ; Tissue Expansion
The purpose of this study was to devise an expanded ischemic flap model and to investigate the role of AMD-3100 (Plerixafor, chemokine receptor 4 inhibitor) in this model by confirming its effect on mobilization of stem cells from the bone marrow. Male Sprague-Dawley rats were used as an animal research model. The mobilization of stem cells from the bone marrow was confirmed in the AMD-3100-treated group. The fractions of endothelial progenitor cells (EPC) and the vascular endothelial growth factor receptor (VEGFR) 2+ cells in the peripheral blood were increased in groups treated with AMD-3100. The expression of vascular endothelial growth factor (VEGF) was increased in response to expansion or AMD injection. The expression of stromal cell derived factor (SDF)-1 and VEGFR2 were increased only in unexpanded flap treated with AMD-3100. Treatment with AMD-3100 increased both the number and area of blood vessels. However, there were no statistically significant differences in the survival area or physiologic microcirculation in rats from the other groups. This endogenous neovascularization induced by AMD-3100 may be a result of the increase in both the area and number of vessels, as well as paracrine augmentation of the expression of VEGF and EPCs. However, the presence of a tissue expander under the flap could block the neovascularization between the flap and the recipient regardless of AMD-3100 treatment and expansion.
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1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Koh, Yoon Woo(고윤우)
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Lew, Dae Hyun(유대현)
Jeong, Hii Sun(정희선)
Tark, Kwan Chul(탁관철)
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