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Identification of Antimicrobial Peptide Hexamers against Oral Pathogens through Rapid Screening of a Synthetic Combinatorial Peptide Library

DC Field Value Language
dc.contributor.author송제선-
dc.contributor.author조경주-
dc.date.accessioned2015-12-28T11:02:51Z-
dc.date.available2015-12-28T11:02:51Z-
dc.date.issued2014-
dc.identifier.issn1226-7155-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/138578-
dc.description.abstractA positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format O(1-6)XXXXX-NH2, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-NH2 (pep-1) and WXXXXN-NH2 (pep-2), and one positioned peptide, RRRWRN-NH2 (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic alpha-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.-
dc.description.statementOfResponsibilityopen-
dc.format.extent169~176-
dc.relation.isPartOfInternational Journal of Oral Biology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleIdentification of Antimicrobial Peptide Hexamers against Oral Pathogens through Rapid Screening of a Synthetic Combinatorial Peptide Library-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Pediatric Dentistry (소아치과학)-
dc.contributor.googleauthorJeseon Song-
dc.contributor.googleauthorKyung Joo Cho-
dc.contributor.googleauthorJoungmok Kim-
dc.contributor.googleauthorJeong Hee Kim-
dc.identifier.doi10.11620/IJOB.2014.39.4.169-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02058-
dc.contributor.localIdA03804-
dc.relation.journalcodeJ01144-
dc.subject.keywordsynthetic antimicrobial peptides-
dc.subject.keywordStreptococcus mutans-
dc.subject.keywordoral pathogens-
dc.subject.keywordhelical wheel-
dc.subject.keywordmembrane disruption-
dc.contributor.alternativeNameSong, Je Seon-
dc.contributor.alternativeNameCho, Kyuong Joo-
dc.contributor.affiliatedAuthorSong, Je Seon-
dc.contributor.affiliatedAuthorCho, Kyuong Joo-
dc.citation.volume39-
dc.citation.number4-
dc.citation.startPage169-
dc.citation.endPage176-
dc.identifier.bibliographicCitationInternational Journal of Oral Biology, Vol.39(4) : 169-176, 2014-
dc.identifier.rimsid38416-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers

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