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Establishment of a mouse melanoma model system for the efficient infection and replication of human adenovirus type 5-based oncolytic virus

Authors
 Sujin Kang  ;  Joo-Hang Kim  ;  So Young Kim  ;  Dongxu Kang  ;  Suyeon Je  ;  Jae J. Song 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.453(3) : 480-485, 2014 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2014
MeSH
Adenoviridae/physiology* ; Animals ; Base Sequence ; Cell Line, Tumor ; DNA Primers ; Male ; Melanoma, Experimental/therapy* ; Mice ; Mice, Inbred C57BL ; Oncolytic Virotherapy* ; Virus Replication*
Keywords
CAR ; E1B-55K ; Mouse melanoma ; Oncolytic adenovirus
Abstract
Due to poor adenoviral infectivity and replication in mouse tumor cell types compared with human tumor cell types, use of human-type adenoviral vectors in mouse animal model systems was limited. Here, we demonstrate enhanced infectivity and productive replication of adenovirus in mouse melanoma cells following introduction of both the Coxsackievirus and adenovirus receptor (CAR) and E1B-55K genes. Introduction of CAR into B16BL6 or B16F10 cells increased the infectivity of GFP-expressing adenovirus; however, viral replication was unaffected. We demonstrated a dramatic increase of adenoviral replication (up to 100-fold) in mouse cells via E1B-55K expression and subsequent viral spreading in mouse tissue. These results reveal for the first time that human adenovirus type 5 (Ad5)-based oncolytic virus can be applied to immunocompetent mouse with the introduction of CAR and E1B-55K to syngenic mouse cell line.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X14017446
DOI
10.1016/j.bbrc.2014.09.107
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Joo Hang(김주항)
Song, Jae Jin(송재진) ORCID logo https://orcid.org/0000-0001-8183-9550
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138392
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