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Establishment of a mouse melanoma model system for the efficient infection and replication of human adenovirus type 5-based oncolytic virus

DC FieldValueLanguage
dc.contributor.author송재진-
dc.contributor.author김주항-
dc.date.accessioned2015-12-28T10:57:34Z-
dc.date.available2015-12-28T10:57:34Z-
dc.date.issued2014-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/138392-
dc.description.abstractDue to poor adenoviral infectivity and replication in mouse tumor cell types compared with human tumor cell types, use of human-type adenoviral vectors in mouse animal model systems was limited. Here, we demonstrate enhanced infectivity and productive replication of adenovirus in mouse melanoma cells following introduction of both the Coxsackievirus and adenovirus receptor (CAR) and E1B-55K genes. Introduction of CAR into B16BL6 or B16F10 cells increased the infectivity of GFP-expressing adenovirus; however, viral replication was unaffected. We demonstrated a dramatic increase of adenoviral replication (up to 100-fold) in mouse cells via E1B-55K expression and subsequent viral spreading in mouse tissue. These results reveal for the first time that human adenovirus type 5 (Ad5)-based oncolytic virus can be applied to immunocompetent mouse with the introduction of CAR and E1B-55K to syngenic mouse cell line.-
dc.description.statementOfResponsibilityopen-
dc.format.extent480~485-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenoviridae/physiology*-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHDNA Primers-
dc.subject.MESHMale-
dc.subject.MESHMelanoma, Experimental/therapy*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHOncolytic Virotherapy*-
dc.subject.MESHVirus Replication*-
dc.titleEstablishment of a mouse melanoma model system for the efficient infection and replication of human adenovirus type 5-based oncolytic virus-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSujin Kang-
dc.contributor.googleauthorJoo-Hang Kim-
dc.contributor.googleauthorSo Young Kim-
dc.contributor.googleauthorDongxu Kang-
dc.contributor.googleauthorSuyeon Je-
dc.contributor.googleauthorJae J. Song-
dc.identifier.doi10.1016/j.bbrc.2014.09.107-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02056-
dc.contributor.localIdA00945-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid25280999-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X14017446-
dc.subject.keywordCAR-
dc.subject.keywordE1B-55K-
dc.subject.keywordMouse melanoma-
dc.subject.keywordOncolytic adenovirus-
dc.contributor.alternativeNameSong, Jae Jin-
dc.contributor.alternativeNameKim, Joo Hang-
dc.contributor.affiliatedAuthorSong, Jae Jin-
dc.contributor.affiliatedAuthorKim, Joo Hang-
dc.rights.accessRightsfree-
dc.citation.volume453-
dc.citation.number3-
dc.citation.startPage480-
dc.citation.endPage485-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.453(3) : 480-485, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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