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Casein kinase II inhibitor enhances production of infectious genotype 1a hepatitis C virus (H77S).

Authors
 Seungtaek Kim  ;  Bora Jin  ;  Sung Hoon Choi  ;  Kwang Hyub Han  ;  Sang Hoon Ahn 
Citation
 PLOS ONE, Vol.9(12) : e113938, 2014 
Journal Title
 PLOS ONE 
Issue Date
2014
MeSH
Antiviral Agents/pharmacology* ; Benzimidazoles/pharmacology ; Casein Kinase II/antagonists & inhibitors* ; Casein Kinase II/chemistry ; Casein Kinase II/genetics ; Cell Line ; Cinnamates/pharmacology ; Genotype* ; Hepacivirus/genetics* ; Hepacivirus/pathogenicity ; Humans ; Molecular Sequence Data ; RNA Interference ; Viral Nonstructural Proteins/chemistry ; Viral Nonstructural Proteins/genetics ; Viral Proteins/chemistry ; Viral Proteins/genetics ; Virus Replication/drug effects
Abstract
Genotype 2a JFH1 virus has substantially contributed to the progress of HCV biology by allowing entire viral life cycle of HCV in cell culture. Using this genotype 2a virus, casein kinase II (CKII) was previously identified as a crucial host factor in virus assembly by phosphorylating NS5A. Since most of the prior studies employed genotype 2a JFH1 or JFH1-based intragenotypic chimera, we used genotype 1a H77S to study virus assembly. CKII inhibition by chemical inhibitors enhanced H77S virus production in contrast to that of JFH1 virus, but genetic inhibition of CKII by siRNA did not change H77S virus titer significantly. The different outcomes from these two approaches of CKII inhibition suggested that nonspecific target kinase of CKII inhibitors plays a role in increasing H77S virus production and both viral and host factors were investigated in this study. Our results emphasize substantial differences among the HCV genotypes that should be considered in both basic research and clinical practices.
Files in This Item:
T201404511.pdf Download
DOI
10.1371/journal.pone.0113938
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Seung Taek(김승택)
Ahn, Sang Hoon(안상훈) ORCID logo https://orcid.org/0000-0002-3629-4624
Choi, Sung Hoon(최성훈)
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138385
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