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Casein kinase II inhibitor enhances production of infectious genotype 1a hepatitis C virus (H77S).

DC FieldValueLanguage
dc.contributor.author김승택-
dc.contributor.author안상훈-
dc.contributor.author최성훈-
dc.contributor.author한광협-
dc.date.accessioned2015-12-28T10:57:23Z-
dc.date.available2015-12-28T10:57:23Z-
dc.date.issued2014-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/138385-
dc.description.abstractGenotype 2a JFH1 virus has substantially contributed to the progress of HCV biology by allowing entire viral life cycle of HCV in cell culture. Using this genotype 2a virus, casein kinase II (CKII) was previously identified as a crucial host factor in virus assembly by phosphorylating NS5A. Since most of the prior studies employed genotype 2a JFH1 or JFH1-based intragenotypic chimera, we used genotype 1a H77S to study virus assembly. CKII inhibition by chemical inhibitors enhanced H77S virus production in contrast to that of JFH1 virus, but genetic inhibition of CKII by siRNA did not change H77S virus titer significantly. The different outcomes from these two approaches of CKII inhibition suggested that nonspecific target kinase of CKII inhibitors plays a role in increasing H77S virus production and both viral and host factors were investigated in this study. Our results emphasize substantial differences among the HCV genotypes that should be considered in both basic research and clinical practices.-
dc.description.statementOfResponsibilityopen-
dc.format.extente113938-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAntiviral Agents/pharmacology*-
dc.subject.MESHBenzimidazoles/pharmacology-
dc.subject.MESHCasein Kinase II/antagonists & inhibitors*-
dc.subject.MESHCasein Kinase II/chemistry-
dc.subject.MESHCasein Kinase II/genetics-
dc.subject.MESHCell Line-
dc.subject.MESHCinnamates/pharmacology-
dc.subject.MESHGenotype*-
dc.subject.MESHHepacivirus/genetics*-
dc.subject.MESHHepacivirus/pathogenicity-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHRNA Interference-
dc.subject.MESHViral Nonstructural Proteins/chemistry-
dc.subject.MESHViral Nonstructural Proteins/genetics-
dc.subject.MESHViral Proteins/chemistry-
dc.subject.MESHViral Proteins/genetics-
dc.subject.MESHVirus Replication/drug effects-
dc.titleCasein kinase II inhibitor enhances production of infectious genotype 1a hepatitis C virus (H77S).-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSeungtaek Kim-
dc.contributor.googleauthorBora Jin-
dc.contributor.googleauthorSung Hoon Choi-
dc.contributor.googleauthorKwang Hyub Han-
dc.contributor.googleauthorSang Hoon Ahn-
dc.identifier.doi10.1371/journal.pone.0113938-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00661-
dc.contributor.localIdA02226-
dc.contributor.localIdA04268-
dc.contributor.localIdA04086-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid25464014-
dc.contributor.alternativeNameKim, Seung Taek-
dc.contributor.alternativeNameAhn, Sang Hoon-
dc.contributor.alternativeNameChoi, Sung Hoon-
dc.contributor.alternativeNameHan, Kwang Hyup-
dc.contributor.affiliatedAuthorKim, Seung Taek-
dc.contributor.affiliatedAuthorAhn, Sang Hoon-
dc.contributor.affiliatedAuthorHan, Kwang Hyup-
dc.contributor.affiliatedAuthorChoi, Sung Hoon-
dc.citation.volume9-
dc.citation.number12-
dc.citation.startPagee113938-
dc.identifier.bibliographicCitationPLOS ONE, Vol.9(12) : e113938, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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