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Ethyl-3,4-dihydroxybenzoate with a dual function of induction of osteogenic differentiation and inhibition of osteoclast differentiation for bone tissue engineering.

 Byeong Ju Kwon  ;  Mi Hee Lee  ;  Min Ah Koo  ;  Jae Jin Han  ;  Jong Chul Park 
 TISSUE ENGINEERING PART A, Vol.20(21~22) : 2975-2984, 2014 
Journal Title
Issue Date
3T3 Cells ; Animals ; Bone Development/drug effects ; Bone Development/physiology ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Cells, Cultured ; Dose-Response Relationship, Drug ; Hydroxybenzoates/administration & dosage* ; Intercellular Signaling Peptides and Proteins/administration & dosage ; Mice ; Osteoblasts/cytology* ; Osteoblasts/drug effects ; Osteoblasts/physiology* ; Osteoclasts/cytology* ; Osteoclasts/drug effects ; Osteoclasts/physiology* ; Osteogenesis/drug effects ; Osteogenesis/physiology* ; Tissue Engineering/methods*
The current approach in biomaterial design of bone implants is to induce in situ regeneration of bone tissue, thus improving integration of the implants and reducing their failure. Therefore, ethyl-3,4-dihydroxybenzoate (EDHB), which stimulates differentiation of osteoblasts and the resultant bone formation, should be studied. In this study, the osteoinductive ability of EDHB in preosteoblasts and human mesenchymal stem cells was examined. EDHB for future use in bone tissue engineering was evaluated by examination of early markers of differentiation (such as alkaline phosphatase [ALP] activity and collagen type I expression) and late markers of osteoblast differentiation (bone nodule formation). As bone remodeling and implant osteointegration depend not only on osteoblast response but also on interaction of the biomaterial with bone-resorbing osteoclasts, differentiation of osteoclasts in response to the compounds was also observed. For in vivo study, alginate gel comprised of EDHB and cells was transplanted into the back subcutis of mice. Our results show that EDHB might have beneficial effects through regulation of both osteoblast and osteoclast differentiation. Therefore, we suggest that EDHB could be a strong candidate for dual regulation to increase osteoblast differentiation and decrease osteoclast differentiation.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Koo, Min-Ah(구민아) ORCID logo https://orcid.org/0000-0002-8671-1131
Kwon, Byeong-Ju(권병주) ORCID logo https://orcid.org/0000-0001-9916-0546
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
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