Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig

Abstract

Purpose: Small intestinal function returns first after surgery, and then thefunction of the stomach returns to normal after postoperative ileus (POI). Theaim of this study was to investigate inflammatory responses in the muscle coatof stomach and small intestine in guinea pig POI model.Materials and Methods: The distance of charcoal migration from pylorus tothe distal intestine was measured. Hematoxylin and eosin (H&E) andimmunohistochemical stain for calprotectin were done from the histologicsections of stomach, jejunum and ileum obtained at 3 and 6 hour afteroperation. Data were compared between sham operation and POI groups.Results: The distance of charcoal migration was significantly reduced in the 3and 6 hour POI groups compared with sham operated groups (p<0.05). OnH&E staining, the degree of inflammation was significantly higher in thestomach of 3 hour POI groups compared with jejunum and ileum of POIgroups or sham operated groups (p<0.05). Calprotectin positive cells weresignificantly increased in the muscle coat of stomach of 3 hour POI groupscompared with jejunum and ileum of POI groups or sham operated groups(p<0.05). There was strong association between the degree of inflammation2and calprotectin positive cells in stomach.Conclusion: Postoperative ileus induced by cecal manipulation significantlyincreased the degree of inflammation and calprotectin positive cells in themuscle coat of stomach as a remote organ. The relevance of degree ofinflammation and the recovery time of ileus should be pursued in the futureresearch.