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S100A8/A9, a DAMP molecule activated by IL-17 and house dust mite is upregulated in atopic dermatitis

Other Titles
 IL-17과 집먼지진드기 항원에 의해 활성화 된 S100A8/A9단백의 아토피피부염에서의 발현 
Authors
 김산 
Issue Date
2011
Description
Dept. of Medicine/석사
Abstract
S100A8/A9 (Calgranulin A/B, Calprotectin), a heterodimer of two calcium-binding proteins originally found in the cytoplasm of neutrophils and membrane of monocytes. It has emerged as an important pro-inflammatory mediator, so called “damage-associated molecular pattern (DAMP)” molecule in acute and chronic inflammation. Our previous proteomics data showed that S100A8/A9 was significantly downregulated after immunotherapy with house dust mite in patients with atopic dermatitis (AD). The purpose of this study was to evaluate S100A8/A9 expression in serum and lesional skin of AD patients, and then to assess S100A8/A9 expression in HaCaT cells and primary human keratinocytes, which were cultured with Th2, Th17 cytokines and house dust mite (HDM) extracts.Compared with healthy controls, serum S100A8/A9 levels were higher in AD patients and correlated with eczema area and severity index (EASI) scores (P<0.01, r2=0.2037). S100A8/A9 was strongly expressed in the upper epidermis of AD tissues by immunofluorescence. IL-17A strongly induced S100A8/A9, and enhanced S100A8/A9 expression in HaCaT cells and human keratinocytes which were cultured with Th2 cytokines. S100A8/A9 mRNA and protein levels were also increased in HaCaT cells and human keratinocytes which were stimulated with Dermatophagoides farinae by time dependent manner. IL-17A also strongly enhanced S100A8/A9 expression in HaCaT cells which were cultured with D. farinae, but Th2 cytokines did not. These results suggest that elevated S100A8/A9 levels of AD patients may reflect the importance of DAMP-associated inflammation, which could be triggered by Th17 cytokines and HDM allergens in AD
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/134147
Appears in Collections:
2. Thesis / Dissertation (학위논문) > 1. College of Medicine (의과대학) > Master's Degree (석사)
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https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000114021
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