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S100A8/A9, a DAMP molecule activated by IL-17 and house dust mite is upregulated in atopic dermatitis

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dc.contributor.author김산-
dc.date.accessioned2015-12-24T08:45:18Z-
dc.date.available2015-12-24T08:45:18Z-
dc.date.issued2011-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/134147-
dc.descriptionDept. of Medicine/석사-
dc.description.abstractS100A8/A9 (Calgranulin A/B, Calprotectin), a heterodimer of two calcium-binding proteins originally found in the cytoplasm of neutrophils and membrane of monocytes. It has emerged as an important pro-inflammatory mediator, so called “damage-associated molecular pattern (DAMP)” molecule in acute and chronic inflammation. Our previous proteomics data showed that S100A8/A9 was significantly downregulated after immunotherapy with house dust mite in patients with atopic dermatitis (AD). The purpose of this study was to evaluate S100A8/A9 expression in serum and lesional skin of AD patients, and then to assess S100A8/A9 expression in HaCaT cells and primary human keratinocytes, which were cultured with Th2, Th17 cytokines and house dust mite (HDM) extracts.Compared with healthy controls, serum S100A8/A9 levels were higher in AD patients and correlated with eczema area and severity index (EASI) scores (P<0.01, r2=0.2037). S100A8/A9 was strongly expressed in the upper epidermis of AD tissues by immunofluorescence. IL-17A strongly induced S100A8/A9, and enhanced S100A8/A9 expression in HaCaT cells and human keratinocytes which were cultured with Th2 cytokines. S100A8/A9 mRNA and protein levels were also increased in HaCaT cells and human keratinocytes which were stimulated with Dermatophagoides farinae by time dependent manner. IL-17A also strongly enhanced S100A8/A9 expression in HaCaT cells which were cultured with D. farinae, but Th2 cytokines did not. These results suggest that elevated S100A8/A9 levels of AD patients may reflect the importance of DAMP-associated inflammation, which could be triggered by Th17 cytokines and HDM allergens in AD-
dc.description.statementOfResponsibilityrestriction-
dc.publisherGraduate School, Yonsei University-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleS100A8/A9, a DAMP molecule activated by IL-17 and house dust mite is upregulated in atopic dermatitis-
dc.title.alternativeIL-17과 집먼지진드기 항원에 의해 활성화 된 S100A8/A9단백의 아토피피부염에서의 발현-
dc.typeThesis-
dc.identifier.urlhttps://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000114021-
dc.identifier.urlhttps://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000114021-
dc.contributor.alternativeNameJin, Shan-
dc.type.localThesis-
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1. College of Medicine (의과대학) > Others (기타) > 2. Thesis

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