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S100A8/A9, a DAMP molecule activated by IL-17 and house dust mite is upregulated in atopic dermatitis
DC Field | Value | Language |
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dc.contributor.author | 김산 | - |
dc.date.accessioned | 2015-12-24T08:45:18Z | - |
dc.date.available | 2015-12-24T08:45:18Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/134147 | - |
dc.description | Dept. of Medicine/석사 | - |
dc.description.abstract | S100A8/A9 (Calgranulin A/B, Calprotectin), a heterodimer of two calcium-binding proteins originally found in the cytoplasm of neutrophils and membrane of monocytes. It has emerged as an important pro-inflammatory mediator, so called “damage-associated molecular pattern (DAMP)” molecule in acute and chronic inflammation. Our previous proteomics data showed that S100A8/A9 was significantly downregulated after immunotherapy with house dust mite in patients with atopic dermatitis (AD). The purpose of this study was to evaluate S100A8/A9 expression in serum and lesional skin of AD patients, and then to assess S100A8/A9 expression in HaCaT cells and primary human keratinocytes, which were cultured with Th2, Th17 cytokines and house dust mite (HDM) extracts.Compared with healthy controls, serum S100A8/A9 levels were higher in AD patients and correlated with eczema area and severity index (EASI) scores (P<0.01, r2=0.2037). S100A8/A9 was strongly expressed in the upper epidermis of AD tissues by immunofluorescence. IL-17A strongly induced S100A8/A9, and enhanced S100A8/A9 expression in HaCaT cells and human keratinocytes which were cultured with Th2 cytokines. S100A8/A9 mRNA and protein levels were also increased in HaCaT cells and human keratinocytes which were stimulated with Dermatophagoides farinae by time dependent manner. IL-17A also strongly enhanced S100A8/A9 expression in HaCaT cells which were cultured with D. farinae, but Th2 cytokines did not. These results suggest that elevated S100A8/A9 levels of AD patients may reflect the importance of DAMP-associated inflammation, which could be triggered by Th17 cytokines and HDM allergens in AD | - |
dc.description.statementOfResponsibility | restriction | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | S100A8/A9, a DAMP molecule activated by IL-17 and house dust mite is upregulated in atopic dermatitis | - |
dc.title.alternative | IL-17과 집먼지진드기 항원에 의해 활성화 된 S100A8/A9단백의 아토피피부염에서의 발현 | - |
dc.type | Thesis | - |
dc.identifier.url | https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000114021 | - |
dc.identifier.url | https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000114021 | - |
dc.contributor.alternativeName | Jin, Shan | - |
dc.type.local | Thesis | - |
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