Bone formation by escherichia coli-expressed rhBMP-2 using MBCP block carrier in rat calvarial defect and ectopic subcutaneous models

Abstract

[한글]

[영문]

E.coli-expressed rhBMP-2 (bone morphogenic protein) is currently being studiedas an alternative to mammalian rhBMP-2. MBCP is a useful carrier for BMPs as itsporous structure, low resorption rate, and favorable osteoconductive and bioactiveproperties allow it to serve as a scaffold for bone forming cells.The purpose of thepresent study was to evaluate bone formation by E.coli-derived rhBMP-2 (ErhBMP-2) using a macroporous biphasic calcium phosphate (MBCP) block carrier in a maleSprague-Dawley rat calvarial defect and ectopic subcutaneous models. Rats weredivided into two groups, a MBCP carrier control and a ErhBMP-2/MBCP group andwere evaluated by histologic and histometric analysis following a 2- or 8- weekhealing interval. For the calvarial defect model, enhanced new bone formation wasobserved in ErhBMP-2/MBCP group. For the ectopic subcutaneous model, few newbone formation was evident in the ErhBMP-2/MBCP group. The MBCP block waseffective as a carrier for E.coli-expressed rhBMP-2 for new bone formation, whichwas stably maintained throughout the healing period. The MBCP block system couldprove to be a useful carrier system for tissue engineering using E.coli-expressedrhBMP-2.