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Hyperosmotic Stimulus Down-regulates 1α, 25-dihydroxyvitamin D₃-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System

Authors
 Yu Shun Tian  ;  Hyun Joo Jeong  ;  Syng-Ill Lee  ;  Byung-Wha Sohn  ;  Dong Min Shin  ;  Jeong-Taeg Seo  ;  Yun-Jung Yoo  ;  Seung-Ho Ohk  ;  Seok Heui Kong  ;  Sang-Do Lee 
Citation
 International Journal of Oral Biology, Vol.30(1) : 23-30, 2005 
Journal Title
 International Journal of Oral Biology 
ISSN
 1226-7155 
Issue Date
2005
MeSH
Hyperosmotic stimulus ; TonEBP ; Osteoblast ; RANKL ; Runx2
Keywords
Hyperosmotic stimulus ; TonEBP ; Osteoblast ; RANKL ; Runx2
Abstract
The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1α, 25-dihydroxyvitamin D3 (1α,25(OH)2D3)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1α,25(OH)2D3-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1α,25(OH)2D3-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1α,25(OH)2D3-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor κB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1α,25(OH)2D3. Knockdown of Runx2 inhibited 1α,25(OH)2D3-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1α,25(OH)2D3-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.
Files in This Item:
T200503625.pdf Download
DOI
10.4196/kjpp.2010.14.3.169
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Ohk, Seung Ho(옥승호)
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Syng Ill(이승일)
Lee, Hyun A(이현아)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114896
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