Cited 1 times in
Hyperosmotic Stimulus Down-regulates 1α, 25-dihydroxyvitamin D₃-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 옥승호 | - |
dc.contributor.author | 유윤정 | - |
dc.contributor.author | 이승일 | - |
dc.contributor.author | 이현아 | - |
dc.contributor.author | 정현주 | - |
dc.date.accessioned | 2015-08-26T16:40:45Z | - |
dc.date.available | 2015-08-26T16:40:45Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114896 | - |
dc.description.abstract | The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1α, 25-dihydroxyvitamin D3 (1α,25(OH)2D3)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1α,25(OH)2D3-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1α,25(OH)2D3-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1α,25(OH)2D3-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor κB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1α,25(OH)2D3. Knockdown of Runx2 inhibited 1α,25(OH)2D3-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1α,25(OH)2D3-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | International Journal of Oral Biology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Hyperosmotic stimulus | - |
dc.subject.MESH | TonEBP | - |
dc.subject.MESH | Osteoblast | - |
dc.subject.MESH | RANKL | - |
dc.subject.MESH | Runx2 | - |
dc.title | Hyperosmotic Stimulus Down-regulates 1α, 25-dihydroxyvitamin D₃-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Yu Shun Tian | - |
dc.contributor.googleauthor | Hyun Joo Jeong | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.googleauthor | Byung-Wha Sohn | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Jeong-Taeg Seo | - |
dc.contributor.googleauthor | Yun-Jung Yoo | - |
dc.contributor.googleauthor | Seung-Ho Ohk | - |
dc.contributor.googleauthor | Seok Heui Kong | - |
dc.contributor.googleauthor | Sang-Do Lee | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02420 | - |
dc.contributor.localId | A02490 | - |
dc.contributor.localId | A02924 | - |
dc.contributor.localId | A03291 | - |
dc.relation.journalcode | J01144 | - |
dc.subject.keyword | Hyperosmotic stimulus | - |
dc.subject.keyword | TonEBP | - |
dc.subject.keyword | Osteoblast | - |
dc.subject.keyword | RANKL | - |
dc.subject.keyword | Runx2 | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Ohk, Seung Ho | - |
dc.contributor.alternativeName | Yoo, Yun Jung | - |
dc.contributor.alternativeName | Lee, Syng Ill | - |
dc.contributor.alternativeName | Lee, Hyun A | - |
dc.contributor.alternativeName | Jung, Hyun Joo | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Ohk, Seung Ho | - |
dc.contributor.affiliatedAuthor | Yoo, Yun Jung | - |
dc.contributor.affiliatedAuthor | Lee, Syng Ill | - |
dc.contributor.affiliatedAuthor | Lee, Hyun A | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 30 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 23 | - |
dc.citation.endPage | 30 | - |
dc.identifier.bibliographicCitation | International Journal of Oral Biology, Vol.30(1) : 23-30, 2005 | - |
dc.identifier.rimsid | 39295 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.