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Helicobacter pylori에 감염된 AGS 세포에서 IL-8 발현에 대한 산화감지 전사인자의 역할

Other Titles
 Role of Oxidant-sensitive Transcription Factors on Helicobacter pylori-induced IL-8 Expression in Gastric Epithelial AGS Cells 
 서정연  ;  김경환  ;  김혜영 
 Korean Journal of Helicobacter and Upper Gastrointestinal Research, Vol.5(2) : 124-133, 2005 
Journal Title
 Korean Journal of Helicobacter and Upper Gastrointestinal Research 
Issue Date
Background/Aims: Oxygen radicals are important regulators in Helicobacter pylori (H. pylori)-induced gastric ulceration and carcinogenesis. IL-8 may be regulated by oxidant-sensitive transcription factors, nuclear-κB (NF-κB), and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori-induced IL-8 expression is regulated by NF-κB and AP-1 in gastric epithelial AGS cells and whether this transcriptional regulation of IL-8 is inhibited by N-acetylcysteine (NAC). Methods: H. pylori was added to the cell at a bacterium/cell ratio of 500:1 in the presence or absence of NAC (1, 5, 10 mM) mRNA expression and protein level was determined by Northern blot analysis and enzyme-linked immunosorbent assay (ELISA), respectively. DNA binding activities of NF-κB and AP-1 were investigated by electrophoretic mobility shift assay (EMSA) and degradation of IκBα protein was monitored by western blot analysis. Results: H. pylori induced the expression of mRNA and protein for IL-8 via activation of NF-κB and AP-1. NF-κB activation accompanied by a decrease in I-κBα in H. pylori-infected AGS cells. NAC inhibited H. pylori-induced activation of transcription factors and IL-8 expression in AGS cells. Conclusions: oxygen radicals induce the activation of NF-κB and AP-1 and IL-8 expression. Antioxidants such as NAC might be useful anti-inflammatory agents by inhibiting activation of transcription factors and decreasing IL-8 production in H. pylori-induced gastric inflammation.
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1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
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