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Helicobacter pylori에 감염된 AGS 세포에서 IL-8 발현에 대한 산화감지 전사인자의 역할
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2015-08-26T16:39:28Z | - |
dc.date.available | 2015-08-26T16:39:28Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1598-415X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114860 | - |
dc.description.abstract | Background/Aims: Oxygen radicals are important regulators in Helicobacter pylori (H. pylori)-induced gastric ulceration and carcinogenesis. IL-8 may be regulated by oxidant-sensitive transcription factors, nuclear-κB (NF-κB), and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori-induced IL-8 expression is regulated by NF-κB and AP-1 in gastric epithelial AGS cells and whether this transcriptional regulation of IL-8 is inhibited by N-acetylcysteine (NAC). Methods: H. pylori was added to the cell at a bacterium/cell ratio of 500:1 in the presence or absence of NAC (1, 5, 10 mM) mRNA expression and protein level was determined by Northern blot analysis and enzyme-linked immunosorbent assay (ELISA), respectively. DNA binding activities of NF-κB and AP-1 were investigated by electrophoretic mobility shift assay (EMSA) and degradation of IκBα protein was monitored by western blot analysis. Results: H. pylori induced the expression of mRNA and protein for IL-8 via activation of NF-κB and AP-1. NF-κB activation accompanied by a decrease in I-κBα in H. pylori-infected AGS cells. NAC inhibited H. pylori-induced activation of transcription factors and IL-8 expression in AGS cells. Conclusions: oxygen radicals induce the activation of NF-κB and AP-1 and IL-8 expression. Antioxidants such as NAC might be useful anti-inflammatory agents by inhibiting activation of transcription factors and decreasing IL-8 production in H. pylori-induced gastric inflammation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 124~133 | - |
dc.relation.isPartOf | Korean Journal of Helicobacter and Upper Gastrointestinal Research | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Helicobacter pylori에 감염된 AGS 세포에서 IL-8 발현에 대한 산화감지 전사인자의 역할 | - |
dc.title.alternative | Role of Oxidant-sensitive Transcription Factors on Helicobacter pylori-induced IL-8 Expression in Gastric Epithelial AGS Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | 서정연 | - |
dc.contributor.googleauthor | 김경환 | - |
dc.contributor.googleauthor | 김혜영 | - |
dc.identifier.doi | OAK-2005-02034 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J02030 | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 5 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 124 | - |
dc.citation.endPage | 133 | - |
dc.identifier.bibliographicCitation | Korean Journal of Helicobacter and Upper Gastrointestinal Research, Vol.5(2) : 124-133, 2005 | - |
dc.identifier.rimsid | 38525 | - |
dc.type.rims | ART | - |
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