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Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films

Authors
 Hyun Joo Son  ;  Dong Wook Han  ;  Jong Chul Park  ;  Jeong Koo Kim  ;  In Seop Lee  ;  Hee Joong Kim  ;  H.H. Kim 
Citation
 Key Engineering Materials, Vol.288~289 : 291-294, 2005 
Journal Title
 Key Engineering Materials 
ISSN
 1013-9826 
Issue Date
2005
MeSH
ECM ; Human Dermal Fibroblast ; PLGA Film ; Tissue Engineering
Keywords
ECM ; Human Dermal Fibroblast ; PLGA Film ; Tissue Engineering
Abstract
In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 µg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 µg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 µg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.
Full Text
http://www.scientific.net/KEM.288-289.291
DOI
10.4028/www.scientific.net/KEM.288-289.291
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Son, Hyun Joo(손현주)
Han, Dong Wook(한동욱)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114840
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