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Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films
DC Field | Value | Language |
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dc.contributor.author | 박종철 | - |
dc.contributor.author | 한동욱 | - |
dc.date.accessioned | 2015-08-26T16:38:38Z | - |
dc.date.available | 2015-08-26T16:38:38Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1013-9826 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114840 | - |
dc.description.abstract | In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 µg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 µg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 µg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | Key Engineering Materials | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | ECM | - |
dc.subject.MESH | Human Dermal Fibroblast | - |
dc.subject.MESH | PLGA Film | - |
dc.subject.MESH | Tissue Engineering | - |
dc.title | Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학) | - |
dc.contributor.googleauthor | Hyun Joo Son | - |
dc.contributor.googleauthor | Dong Wook Han | - |
dc.contributor.googleauthor | Jong Chul Park | - |
dc.contributor.googleauthor | Jeong Koo Kim | - |
dc.contributor.googleauthor | In Seop Lee | - |
dc.contributor.googleauthor | Hee Joong Kim | - |
dc.contributor.googleauthor | H.H. Kim | - |
dc.identifier.doi | 10.4028/www.scientific.net/KEM.288-289.291 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01662 | - |
dc.contributor.localId | A02001 | - |
dc.contributor.localId | A04275 | - |
dc.relation.journalcode | J01939 | - |
dc.identifier.pmid | 10.4028/www.scientific.net/KEM.288-289.291 | - |
dc.identifier.url | http://www.scientific.net/KEM.288-289.291 | - |
dc.subject.keyword | ECM | - |
dc.subject.keyword | Human Dermal Fibroblast | - |
dc.subject.keyword | PLGA Film | - |
dc.subject.keyword | Tissue Engineering | - |
dc.contributor.alternativeName | Park, Jong Chul | - |
dc.contributor.alternativeName | Han, Dong Wook | - |
dc.contributor.affiliatedAuthor | Park, Jong Chul | - |
dc.contributor.affiliatedAuthor | Han, Dong Wook | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 288~289 | - |
dc.citation.startPage | 291 | - |
dc.citation.endPage | 294 | - |
dc.identifier.bibliographicCitation | Key Engineering Materials, Vol.288~289 : 291-294, 2005 | - |
dc.identifier.rimsid | 38509 | - |
dc.type.rims | ART | - |
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