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NADPH oxidase and apoptosis in cerulein-stimulated pancreatic acinar AR42J cells

Authors
 Ji Hoon Yu  ;  Joo Weon Lim  ;  Hyeyoung Kim  ;  Tomohiro Morio  ;  Kyung Hwan Kim 
Citation
 FREE RADICAL BIOLOGY AND MEDICINE, Vol.39(5) : 590-602, 2005 
Journal Title
 FREE RADICAL BIOLOGY AND MEDICINE 
ISSN
 0891-5849 
Issue Date
2005
MeSH
Animals ; Apoptosis* ; Blotting, Western ; Calcium/metabolism ; Caspase 3 ; Caspases/metabolism ; Cell Count ; Cell Line ; Cell Membrane/metabolism ; Cell Survival ; Ceruletide/pharmacology* ; Chelating Agents/pharmacology ; Cytosol/metabolism ; DNA Fragmentation ; Dose-Response Relationship, Drug ; Egtazic Acid/analogs & derivatives ; Egtazic Acid/pharmacology ; In Situ Nick-End Labeling ; NADPH Oxidases/metabolism* ; Oligonucleotides, Antisense/chemistry ; Pancreas/enzymology ; Pancreas/pathology* ; Phosphoproteins/metabolism ; Protein Transport ; Rats ; Reactive Oxygen Species ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors ; Transfection
Keywords
NADPH oxidase ; Apoptosis ; Reactive oxygen species ; Pancreatic acinar cells
Abstract
Apoptosis linked to oxidative stress has been implicated in pancreatitis. We investigated whether NADPH oxidase mediates apoptosis in cerulein-stimulated pancreatic acinar AR42J cells. We report here that cerulein treatment resulted in the activation of NADPH oxidase, as determined by ROS production, translocation of cytosolic subunits p47phox and p67phox to the membrane, and interaction between NADPH oxidase subunits. Cerulein induced Ca2+ oscillation, the expression of apoptotic genes p53 and bax, and apoptotic indices (DNA fragmentation, TUNEL staining, caspase 3 activity, decrease in cell viability) in AR42J cells. Treatment with a Ca2+ chelator, BAPTA-AM, or transfection with antisense oligonucleotides for NADPH oxidase subunits p22phox and p47phox inhibited cerulein-induced ROS production, translocation of NADPH oxidase cytosolic subunits p47phox and p67phox to the membrane, and the expression of apoptotic genes and apoptotic indices, as compared to the cells without treatment and those transfected with the corresponding sense oligonucleotides. These results indicate that NADPH oxidase may mediate ROS-induced apoptosis in pancreatic acinar cells in a Ca2+-dependent manner.
Full Text
http://www.sciencedirect.com/science/article/pii/S089158490500211X
DOI
10.1016/j.freeradbiomed.2005.04.019
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Gastroenterology (소화기병연구소) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
Lim, Joo Weon(임주원)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114800
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