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p53 qnd p21 waf1/cip1 in hepatitis B virus related hepatocarcinogenesis

 Young Nyun Park  ;  Kwang Jo Chae  ;  Chanil Park  ;  Woo Jung Lee  ;  Kwan Sik Lee  ;  Bong-Kyeong Oh  ;  Kye Won Kwon 
 HEPATO-GASTROENTEROLOGY, Vol.50(53) : 1292-1296, 2003 
Journal Title
Issue Date
Carcinoma, Hepatocellular/genetics* ; Carcinoma, Hepatocellular/virology ; Cyclin-Dependent Kinase Inhibitor p21 ; Cyclins/metabolism* ; Female ; Gene Expression Regulation, Neoplastic/physiology* ; Hepatitis B/complications ; Humans ; Immunohistochemistry ; Liver Neoplasms/genetics* ; Liver Neoplasms/virology ; Male ; Middle Aged ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Suppressor Protein p53/metabolism*
BACKGROUND/AIMS: This study was undertaken to determine the expression p21WAF1/CIP1 and p53, the main regulator of G1 restriction point, in hepatitis B virus related hepatocarcinogenesis and their role in regulation of cell dynamics. METHODOLOGY: Immunohistochemistry for p21WAF1/CIP1, p53 and Ki-67 and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling were preformed in 8 low grade dysplastic nodules, 7 high grade dysplastic nodules, 58 hepatocellular carcinomas, and 62 chronic hepatitis/cirrhosis. In 33 hepatocellular carcinomas with fresh frozen tissue, p21WAF1/CIP1 mRNA was studied by reverse transcriptase polymerase chain reaction. RESULTS: Immunostaining for p21WAF1/CIP1 and p53 was positive in 28% and 38% of hepatocellular carcinomas and higher in poorly differentiated hepatocellular carcinomas, whereas it was negative in all of chronic hepatitis/cirrhosis and dysplastic nodules. In hepatocellular carcinomas with p21WAF1/CIP1 mRNA amplified, 31% showed the protein expression indicating post-transcriptional regulation. The rate of apoptosis and proliferation showed significant correlation with p53 status, however not with p21WAF1/CIP1 in hepatocellular carcinomas. Induction of p21WAF1/CIP1 was regulated by both p53 dependent and independent pathway. CONCLUSIONS: In hepatitis B virus related hepatocarcinogenesis, p21WAF1/CIP1 and p53 are suggested to be involved in late stage of tumor progression rather than in early stage of dysplastic nodules and p53 is considered to be the main regulator of the cell dynamics.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
Park, Chan Il(박찬일)
Oh, Bong Kyeong(오봉경)
Lee, Kwan Sik(이관식) ORCID logo https://orcid.org/0000-0002-3672-1198
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