2 227

Cited 0 times in

Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells

Authors
 Myung-Jin Park  ;  In-Chul Park  ;  Seok-Il Hong  ;  Toshino Kuroki  ;  Bum-Sang Shim  ;  Seung-Hoon Lee  ;  Yun-Sil Lee  ;  Chang-Hun Rhee  ;  Young-Joon Hong  ;  Jae-Young Lee  ;  Sang-Hyeok Woo  ;  Hyung-Chan Lee 
Citation
 INTERNATIONAL JOURNAL OF ONCOLOGY, Vol.22(1) : 137-143, 2003 
Journal Title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN
 1019-6439 
Issue Date
2003
Abstract
In the present study, phorbol 12-myristate 13-acetate (PMA) was found to increase secretion of matrix metalloproteinase (MMP)-9 and in vitro invasion in bovine capillary endothelial (BCE) cells, which were blocked by specific inhibitors of protein kinase C (PKC). To elucidate molecular mechanisms involved, we studied the effect of PMA on the activation of mitogen activated protein kinases (MAPKs), and found that PMA activated extracellular signal-regulated kinase (ERK)1/2 and PD98059, a specific inhibitor of MAPK kinase, significantly reduced PMA-induced MMP-9 secretion as well as in vitro invasion of BCE cells. Treatment of safingol, a specific PKC-α inhibitor, and introduction of antisense PKC-α into these cells reduced the secretion of MMP-9 and activation of ERK1/2 by PMA. Furthermore, we employed adenoviral PKC-α and found that weak PMA stimulation (5 ng/ml) enhanced ERK1/2 activation and MMP-9 secretion in these cells. Therefore, we strongly suggest that PKC-α, partly at least, have a crucial role in MMP-9 secretion and invasion of BCE cells which are mediated via ERK1/2 signaling pathway.
Full Text
http://www.spandidos-publications.com/ijo/22/1/137
DOI
10.3892/ijo.22.1.137
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Sil(이윤실)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113698
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links