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Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells
DC Field | Value | Language |
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dc.contributor.author | 이윤실 | - |
dc.date.accessioned | 2015-07-15T16:50:18Z | - |
dc.date.available | 2015-07-15T16:50:18Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113698 | - |
dc.description.abstract | In the present study, phorbol 12-myristate 13-acetate (PMA) was found to increase secretion of matrix metalloproteinase (MMP)-9 and in vitro invasion in bovine capillary endothelial (BCE) cells, which were blocked by specific inhibitors of protein kinase C (PKC). To elucidate molecular mechanisms involved, we studied the effect of PMA on the activation of mitogen activated protein kinases (MAPKs), and found that PMA activated extracellular signal-regulated kinase (ERK)1/2 and PD98059, a specific inhibitor of MAPK kinase, significantly reduced PMA-induced MMP-9 secretion as well as in vitro invasion of BCE cells. Treatment of safingol, a specific PKC-α inhibitor, and introduction of antisense PKC-α into these cells reduced the secretion of MMP-9 and activation of ERK1/2 by PMA. Furthermore, we employed adenoviral PKC-α and found that weak PMA stimulation (5 ng/ml) enhanced ERK1/2 activation and MMP-9 secretion in these cells. Therefore, we strongly suggest that PKC-α, partly at least, have a crucial role in MMP-9 secretion and invasion of BCE cells which are mediated via ERK1/2 signaling pathway. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 137~143 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Protein kinase C-α activation by phorbol ester induces secretion of gelatinase B/MMP-9 through ERK 1/2 pathway in capillary endothelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Myung-Jin Park | - |
dc.contributor.googleauthor | In-Chul Park | - |
dc.contributor.googleauthor | Seok-Il Hong | - |
dc.contributor.googleauthor | Toshino Kuroki | - |
dc.contributor.googleauthor | Bum-Sang Shim | - |
dc.contributor.googleauthor | Seung-Hoon Lee | - |
dc.contributor.googleauthor | Yun-Sil Lee | - |
dc.contributor.googleauthor | Chang-Hun Rhee | - |
dc.contributor.googleauthor | Young-Joon Hong | - |
dc.contributor.googleauthor | Jae-Young Lee | - |
dc.contributor.googleauthor | Sang-Hyeok Woo | - |
dc.contributor.googleauthor | Hyung-Chan Lee | - |
dc.identifier.doi | 10.3892/ijo.22.1.137 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03021 | - |
dc.relation.journalcode | J01141 | - |
dc.identifier.eissn | 1791-2423 | - |
dc.identifier.pmid | 10.3892/ijo.22.1.137 | - |
dc.identifier.url | http://www.spandidos-publications.com/ijo/22/1/137 | - |
dc.contributor.alternativeName | Lee, Yun Sil | - |
dc.contributor.affiliatedAuthor | Lee, Yun Sil | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 22 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 137 | - |
dc.citation.endPage | 143 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF ONCOLOGY, Vol.22(1) : 137-143, 2003 | - |
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