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Optimization of vesicular stomatitis virus-G pseudotyped feline immunodeficiency virus vector for minimized cytotoxicity with efficient gene transfer

Authors
 Jae Jin Song  ;  Boyoung Lee  ;  Jin Woo Chang  ;  Joo-Hang Kim  ;  Yunhee Kim Kwon  ;  Heuiran Lee 
Citation
 VIRUS RESEARCH, Vol.93(1) : 25-30, 2003 
Journal Title
VIRUS RESEARCH
ISSN
 0168-1702 
Issue Date
2003
MeSH
Animals ; Cats ; Cell Line ; Cell Survival/genetics ; Cell Survival/physiology* ; Gene Transfer Techniques ; Genetic Vectors ; Humans ; Immunodeficiency Virus, Feline/physiology* ; Membrane Glycoproteins/physiology* ; Vesicular stomatitis Indiana virus/classification ; Vesicular stomatitis Indiana virus/genetics ; Vesicular stomatitis Indiana virus/physiology* ; Viral Envelope Proteins/physiology*
Keywords
FIV-based vector ; Gag ; Vif ; Transduction efficiency ; Cytotoxicity
Abstract
FIV-based lentiviral vector has shown a unique opportunity as an efficient gene delivery vehicle, especially to nondividing human cells. Here, we genetically reconstructed the FIV-based vector by serially deleting residual virus genes of gag and vif, leading to minimized cytotoxicity together with efficient virus production and gene transfer. The modified FIV- based vector was generated by transiently transfecting 293T cells with three plasmids of the gene transfer vector with minimal gag region, the packaging plasmid without vif and the VSV-G-expressing plasmid. The vector was routinely generated as many as 1×107 transducing particles per ml and easily concentrated by simple centrifugation. The cytotoxic effect significantly decreased in sensitive cells to FIV infection even at high multiplicity of infection (MOI), such as 500. Moreover, the transduction efficiency was consistently retained after cell cycle was arrested in a variety of human cells. Taken together, our results suggest that the modified VSV-G pseudotyped FIV-based vector efficiently transduce dividing and nondividing human cells with minimal cytotoxicity.
Full Text
http://www.sciencedirect.com/science/article/pii/S0168170203000479
DOI
10.1016/S0168-1702(03)00047-9
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Hang(김주항)
Song, Jae Jin(송재진) ORCID logo https://orcid.org/0000-0001-8183-9550
Chang, Jin Woo(장진우) ORCID logo https://orcid.org/0000-0002-2717-0101
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113582
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