1 476

Cited 14 times in

Proteomic and transcriptomic analysis of interleukin-1ß treated lung carcinoma cell line

Authors
 Chang-Hoon Kim  ;  Do Kyun Kim  ;  Je Kyung Seong  ;  Joo-Heon Yoon  ;  Seung Yong Hwang  ;  Ja Seok Koo  ;  Janghoon Chi  ;  Kyoung Seob Song  ;  Kun Ho Choi  ;  Seung Jin Choi 
Citation
 PROTEOMICS, Vol.3(12) : 2454-2471, 2003 
Journal Title
 PROTEOMICS 
ISSN
 1615-9853 
Issue Date
2003
MeSH
Carcinoma/immunology* ; Carcinoma/metabolism ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Regulation, Neoplastic/drug effects* ; Humans ; Interleukin-1/immunology ; Interleukin-1/pharmacology* ; Lung Neoplasms/immunology* ; Lung Neoplasms/metabolism ; Mass Spectrometry ; Mucins/metabolism* ; Oligonucleotide Array Sequence Analysis ; Tumor Cells, Cultured
Keywords
cDNA microarray ; Hypersecretion ; Mucin ; Mucus ; Two-dimensional gel electro-phoresis
Abstract
Mucin hypersecretion is one of the main symptoms of inflammatory disease in the respiratory tract. We previously reported that the pleiotypic pro-inflammatory cytokine, interleukin (IL)-1β, plays a significant role in respiratory tract inflammation by inducing mucins. However, the molecular mechanism for mucin hypersecretion in the respiratory tract remains still unclear. In order to understand the mechanisms of mucin hypersecretion in the airway epithelium, the differentially expressed proteins and genes in the lung mucoepidermoid carcinoma cell line (NCI-H292 cells), which were treated for 6 and 24 hours with IL-1β (10 ng/mL) were identified using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) proteomics and cDNA microarray analysis (8.6K). In the 2-D PAGE, eight differentially expressed proteins and 14 post-translational modification proteins were identified at 6 and 24 hours after the IL-1β-treatment. Four hundred and thirteen genes (6.6%) and 115 genes (2.0%) were differentially expressed, respectively, at 6 and 24 hours after the IL-1β-treatment by microarray analysis. The differentially expressed genes and proteins that were regulated by the IL-1β-treatment were mostly in the metabolic pathway rather than in the regulatory pathway. These results clearly show that the transcript levels have little value in predicting the extent of protein expression.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/pmic.200300643/abstract
DOI
10.1002/pmic.200300643
Appears in Collections:
5. Research Institutes (연구소) > Airway Hucus Institute (기도점액연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Song, Kyoung Seob(송경섭)
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113577
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links