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Protective effects of green tea polyphenol against reactive oxygen species-induced oxidative stress in cultured rat calvarial osteoblast

 Y.H. Park  ;  D.-W. Han  ;  J.-C. Park  ;  B.K. Cho  ;  S.-H. Hyon  ;  G.H. Ryu  ;  H. Suh 
 Cell Biology and Toxicology, Vol.19(5) : 325-337, 2003 
Journal Title
 Cell Biology and Toxicology 
Issue Date
green tea polyphenols ; oxidative stress ; rat calvarial osteoblasts ; reactive oxygen species ; viability
green tea polyphenols ; oxidative stress ; rat calvarial osteoblasts ; reactive oxygen species ; viability
The injurious effects of reactive oxygen species on osteoblasts and the potential protective role played by green tea polyphenols (GtPP) were investigated using primarily cultured rat calvarial osteoblasts. Oxidative stress was induced in cultured osteoblasts, either by adding 100 mmol/L H2O2 or by the action of 40 U/L xanthine oxidase (XO) in the presence of xanthine (250 μmol/L). After incubation, the cellular viability, function and morphology were evaluated. Both treatments produced a significant reduction in osteoblast viability, as assessed by a two-colored fluorescence staining method combined with flow cytometric analysis and MTT assay. A significant reduction in the alkaline phosphatase activity was observed after H2O2 addition, whereas XO did not have the same effect. On the microscopic observations, the morphological changes and intracellular ultrastructural damages were remarkably induced by both treatments. The H2O2-induced alterations were prevented by pre-incubating the osteoblasts with 200 μg/ml GtPP for 1 h. When the oxidative stress was induced by XO, the cellular viability and morphology was also maintained at the same polyphenol concentration. These results demonstrate that GtPP can act as a biological antioxidant in a cell culture experimental model and protect cells from oxidative stress-induced toxicity.
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1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
Cho, Bum Koo(조범구)
Han, Dong Wook(한동욱)
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