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NF-κB and Bcl-2 in Helicobacter pylori-Induced Apoptosis in Gastric Epithelial Cells

Authors
 SANG HUI CHU  ;  JOO WEON LIM  ;  HYEYOUNG KIM  ;  KYUNG HWAN KIM 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 568-572, 2003 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2003
MeSH
NF-κB ; Bcl-2 ; Helicobacter pylori ; apoptosis ; gastric epithelialcells
Keywords
NF-κB ; Bcl-2 ; Helicobacter pylori ; apoptosis ; gastric epithelialcells
Abstract
Helicobacter pylori (H. pylori) has been considered as an important pathogen of gastroduodenal inflammation and gastric carcinogenesis. However, the pathogenic mechanisms including H. pylori-induced apoptosis and subsequent molecular mechanisms have not been clarified yet. The present study examined the role of Bcl-2 and its relation to NF-κB in H. pylori-induced apoptosis in human gastric epithelial AGS cells. AGS cells were cultured in the presence of H. pylori, at a bacterium/cell ratio of 300:1, for the determination of apoptosis, NF-κB activation with IkBa degradation, and Bcl-2 level. AGS cells were transfected with a control vector (pCMV cells) or a full-length human Bcl-2 expression vector (Bcl-2 cells). H. pylori-induced apoptosis and NF-κB activation were compared in the wild-type cells and the transfected cells. As a result, H. pylori increased apoptotic cells with chromatin condensation and reduced Bcl-2 levels, which were accompanied with NF-κB activation. H. pylori induced sixfold increase in the number of apoptotic cells in wild-type cells and pCMV cells. H. pylori-induced increment of apoptotic cells were relatively lower in Bcl-2 cells than pCMV cells. H. pylori-induced NF-κB activation and IkBa degradation were not different in the wild-type cells, pCMV cells, and Bcl-2 cells. In conclusion, the reduced gastric Bcl-2 level may be the main pathogenic mechanism of H. pylori-induced apoptosis in gastric epithelial cells.
Full Text
http://onlinelibrary.wiley.com/doi/10.1196/annals.1299.106/abstract
DOI
10.1196/annals.1299.106
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
3. College of Nursing (간호대학) > Dept. of Nursing (간호학과) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
Lim, Joo Weon(임주원)
Chu, Sang Hui(추상희) ORCID logo https://orcid.org/0000-0001-6877-5599
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113309
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