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N-CoR Mediates DNA Methylation-Dependent Repression through a Methyl CpG Binding Protein Kaiso

Authors
 Ho-Geun Yoon  ;  Doug W. Chan  ;  Jiemin Wong  ;  Jun Qin  ;  Albert B. Reynolds 
Citation
 MOLECULAR CELL, Vol.12(3) : 723-734, 2003 
Journal Title
 MOLECULAR CELL 
ISSN
 1097-2765 
Issue Date
2003
MeSH
Animals ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; CpG Islands/genetics* ; DNA Methylation* ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism* ; DNA-Cytosine Methylases/genetics ; DNA-Cytosine Methylases/metabolism ; Eukaryotic Cells/metabolism ; Genes, Regulator/genetics* ; HeLa Cells ; Histone Deacetylases/genetics ; Histone Deacetylases/metabolism ; Histones/genetics ; Histones/metabolism ; Humans ; Lysine/genetics ; Lysine/metabolism ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism* ; Nuclear Receptor Co-Repressor 1 ; Promoter Regions, Genetic/genetics ; Repressor Proteins/genetics ; Repressor Proteins/metabolism* ; Transcription Factors/genetics ; Transcription Factors/metabolism*
Keywords
14527417
Abstract
The identification and characterization of molecular mechanisms utilized by cells to mediate transcriptional repression at methylated loci are fundamental to understanding the biological consequences of DNA methylation. Here we demonstrate that Kaiso, a methyl CpG binding protein belonging to the BTB/POZ family of transcription factors, is a component of the human N-CoR complex. In vitro, the Kaiso/N-CoR complex binds specific CpG-rich sequences in a methylation-dependent manner. In vivo, Kaiso targets the N-CoR complex to the MTA2 gene promoter in a methylation-dependent manner. Importantly, we demonstrate that Kaiso is required for transcriptional repression of the methylated MTA2 locus. Furthermore, this repression also requires a functional N-CoR deacetylase complex, which brings about histone hypoacetylation and methylation of H3 lysine 9 to the MTA2 locus. Thus, our data demonstrate a critical role for a methyl CpG binding protein in mediating DNA methylation-dependent repression and reveal the mechanism by which it represses transcription.
Full Text
http://www.sciencedirect.com/science/article/pii/S1097276503003174
DOI
OAK-2003-00144
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113304
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