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N-CoR Mediates DNA Methylation-Dependent Repression through a Methyl CpG Binding Protein Kaiso

DC FieldValueLanguage
dc.contributor.author윤호근-
dc.date.accessioned2015-07-15T16:38:29Z-
dc.date.available2015-07-15T16:38:29Z-
dc.date.issued2003-
dc.identifier.issn1097-2765-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113304-
dc.description.abstractThe identification and characterization of molecular mechanisms utilized by cells to mediate transcriptional repression at methylated loci are fundamental to understanding the biological consequences of DNA methylation. Here we demonstrate that Kaiso, a methyl CpG binding protein belonging to the BTB/POZ family of transcription factors, is a component of the human N-CoR complex. In vitro, the Kaiso/N-CoR complex binds specific CpG-rich sequences in a methylation-dependent manner. In vivo, Kaiso targets the N-CoR complex to the MTA2 gene promoter in a methylation-dependent manner. Importantly, we demonstrate that Kaiso is required for transcriptional repression of the methylated MTA2 locus. Furthermore, this repression also requires a functional N-CoR deacetylase complex, which brings about histone hypoacetylation and methylation of H3 lysine 9 to the MTA2 locus. Thus, our data demonstrate a critical role for a methyl CpG binding protein in mediating DNA methylation-dependent repression and reveal the mechanism by which it represses transcription.-
dc.description.statementOfResponsibilityopen-
dc.format.extent723~734-
dc.relation.isPartOfMOLECULAR CELL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCarrier Proteins/genetics-
dc.subject.MESHCarrier Proteins/metabolism-
dc.subject.MESHCpG Islands/genetics*-
dc.subject.MESHDNA Methylation*-
dc.subject.MESHDNA-Binding Proteins/genetics-
dc.subject.MESHDNA-Binding Proteins/metabolism*-
dc.subject.MESHDNA-Cytosine Methylases/genetics-
dc.subject.MESHDNA-Cytosine Methylases/metabolism-
dc.subject.MESHEukaryotic Cells/metabolism-
dc.subject.MESHGenes, Regulator/genetics*-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHistone Deacetylases/genetics-
dc.subject.MESHHistone Deacetylases/metabolism-
dc.subject.MESHHistones/genetics-
dc.subject.MESHHistones/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHLysine/genetics-
dc.subject.MESHLysine/metabolism-
dc.subject.MESHNuclear Proteins/genetics-
dc.subject.MESHNuclear Proteins/metabolism*-
dc.subject.MESHNuclear Receptor Co-Repressor 1-
dc.subject.MESHPromoter Regions, Genetic/genetics-
dc.subject.MESHRepressor Proteins/genetics-
dc.subject.MESHRepressor Proteins/metabolism*-
dc.subject.MESHTranscription Factors/genetics-
dc.subject.MESHTranscription Factors/metabolism*-
dc.titleN-CoR Mediates DNA Methylation-Dependent Repression through a Methyl CpG Binding Protein Kaiso-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorHo-Geun Yoon-
dc.contributor.googleauthorDoug W. Chan-
dc.contributor.googleauthorJiemin Wong-
dc.contributor.googleauthorJun Qin-
dc.contributor.googleauthorAlbert B. Reynolds-
dc.identifier.doiOAK-2003-00144-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02625-
dc.relation.journalcodeJ02256-
dc.identifier.eissn1097-4164-
dc.identifier.pmid14527417-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1097276503003174-
dc.subject.keyword14527417-
dc.contributor.alternativeNameYoon, Ho Geun-
dc.contributor.affiliatedAuthorYoon, Ho Geun-
dc.rights.accessRightsnot free-
dc.citation.volume12-
dc.citation.number3-
dc.citation.startPage723-
dc.citation.endPage734-
dc.identifier.bibliographicCitationMOLECULAR CELL, Vol.12(3) : 723-734, 2003-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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