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The Effect of p38 Mitogen-Activated Protein Kinase on Mucin Gene Expression and Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cells

Authors
 HYEYOUNG KIM  ;  JI HYE SEO  ;  KYUNG HWAN KIM 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 90-94, 2003 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2003
MeSH
Adenocarcinoma ; Apoptosis/physiology* ; Cell Line, Tumor ; Gastric Mucins/genetics* ; Gastric Mucosa/microbiology ; Gastric Mucosa/pathology ; Gastric Mucosa/physiopathology ; Gene Expression Regulation, Neoplastic* ; Helicobacter Infections/pathology* ; Helicobacter pylori* ; Humans ; Mitogen-Activated Protein Kinases/metabolism* ; Stomach Neoplasms ; p38 Mitogen-Activated Protein Kinases
Keywords
15033700
Abstract
The loss of mucus coat continuity and apoptosis have been shown in Helicobacter pylori (H. pylori)-infected gastric tissues. Blockade of p38 mitogen-activated kinase (MAPK) produced reversal in the LPS-induced reduction in mucin synthesis and apoptosis in gastric epithelial cells. This study investigates whether H. pylori induces apoptosis, alterations in mucin gene (MUC) expression, and p38 MAPK activation in human gastric epithelial AGS cells. After treatment of AGS cells with H. pylori at the ratio of 1:300, apoptosis was determined by DNA fragmentation and DNA laddering. MUC expression was assessed by RT-PCR. p38 MAPK activation and mucin protein level, using anti-mucin antibody for MUC5/6, were determined by Western blot analysis. As a result, H. pylori induced apoptosis and loss of mucin, which was supported by reduced mRNA expression of MUC5AC by H. pylori in AGS cells. MUC7/8 expression and p38 MAPK activation were induced in H. pylori-infected AGS cells. In conclusion, H. pylori induces p38 MAPK activation, wh3.ich may be the underlying mechanism of alterations in MUC expression and apoptosis in gastric epithelial cells.
Full Text
http://onlinelibrary.wiley.com/doi/10.1196/annals.1299.014/abstract
DOI
OAK-2003-00038
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113198
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