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The Effect of p38 Mitogen-Activated Protein Kinase on Mucin Gene Expression and Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cells

DC FieldValueLanguage
dc.contributor.author김경환-
dc.date.accessioned2015-07-15T16:35:18Z-
dc.date.available2015-07-15T16:35:18Z-
dc.date.issued2003-
dc.identifier.issn0077-8923-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113198-
dc.description.abstractThe loss of mucus coat continuity and apoptosis have been shown in Helicobacter pylori (H. pylori)-infected gastric tissues. Blockade of p38 mitogen-activated kinase (MAPK) produced reversal in the LPS-induced reduction in mucin synthesis and apoptosis in gastric epithelial cells. This study investigates whether H. pylori induces apoptosis, alterations in mucin gene (MUC) expression, and p38 MAPK activation in human gastric epithelial AGS cells. After treatment of AGS cells with H. pylori at the ratio of 1:300, apoptosis was determined by DNA fragmentation and DNA laddering. MUC expression was assessed by RT-PCR. p38 MAPK activation and mucin protein level, using anti-mucin antibody for MUC5/6, were determined by Western blot analysis. As a result, H. pylori induced apoptosis and loss of mucin, which was supported by reduced mRNA expression of MUC5AC by H. pylori in AGS cells. MUC7/8 expression and p38 MAPK activation were induced in H. pylori-infected AGS cells. In conclusion, H. pylori induces p38 MAPK activation, wh3.ich may be the underlying mechanism of alterations in MUC expression and apoptosis in gastric epithelial cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent90~94-
dc.relation.isPartOfANNALS OF THE NEW YORK ACADEMY OF SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenocarcinoma-
dc.subject.MESHApoptosis/physiology*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHGastric Mucins/genetics*-
dc.subject.MESHGastric Mucosa/microbiology-
dc.subject.MESHGastric Mucosa/pathology-
dc.subject.MESHGastric Mucosa/physiopathology-
dc.subject.MESHGene Expression Regulation, Neoplastic*-
dc.subject.MESHHelicobacter Infections/pathology*-
dc.subject.MESHHelicobacter pylori*-
dc.subject.MESHHumans-
dc.subject.MESHMitogen-Activated Protein Kinases/metabolism*-
dc.subject.MESHStomach Neoplasms-
dc.subject.MESHp38 Mitogen-Activated Protein Kinases-
dc.titleThe Effect of p38 Mitogen-Activated Protein Kinase on Mucin Gene Expression and Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorHYEYOUNG KIM-
dc.contributor.googleauthorJI HYE SEO-
dc.contributor.googleauthorKYUNG HWAN KIM-
dc.identifier.doiOAK-2003-00038-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00311-
dc.relation.journalcodeJ00181-
dc.identifier.eissn1749-6632-
dc.identifier.pmid15033700-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1196/annals.1299.014/abstract-
dc.subject.keyword15033700-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.rights.accessRightsnot free-
dc.citation.volume1010-
dc.citation.startPage90-
dc.citation.endPage94-
dc.identifier.bibliographicCitationANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 90-94, 2003-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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