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Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells

Authors
 JANGWON LEE  ;  JEONGHUN SEO  ;  KYUNG HWAN KIM  ;  JAE BOCK CHUNG  ;  HYEYOUNG KIM 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 104-108, 2003 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2003
MeSH
Animals ; Apoptosis/drug effects* ; Apoptosis/immunology ; Ceruletide/pharmacology* ; Cytokines/genetics* ; Gene Expression Regulation/drug effects ; Genes, ras ; Pancreas/cytology* ; Pancreas/drug effects ; Pancreas/physiology ; Rats ; Signal Transduction/drug effects ; Signal Transduction/physiology* ; Transfection
Keywords
15033703
Abstract
The signaling pathways mediating cytokine production and apoptosis in pancreatic acinar cells have not been fully understood. We investigated the signal transduction of cerulein-induced cytokine IL-6 expression and apoptosis in pancreatic acinar AR42J cells. The wild-type cells and the cells transfected with mutant genes for signaling molecules (such as transcription factor NF-kappaB and AP-1) and ras, the upstream signal for mitogen-activated protein kinase (MAPK), were used. IL-6 level was determined in the medium by enzyme-linked immunosorbent assay (ELISA). Apoptosis was determined by viable cell counts and DNA fragmentation. Wild-type cells and transfected cells with control vector (pcDNA), IkappaB mutant gene (IkappaB mt), H-ras mutant gene (Ras N17), or c-jun dominant negative gene (TAM-67) were treated with cerulein for 24 hours. As a result, cerulein induced IL-6 expression time-dependently at 10(-8) M, and apoptosis dose-dependently at 24 h in the wild-type cells. Cerulein-induced IL-6 expression (at 10(-8) M) and apoptosis (at 10(-7) M) were inhibited in the transfected cells with mutant gene (IkappaB mt, Ras N17, TAM-67) as compared to pcDNA cells and the wild-type cells. In conclusion, cerulein induces cytokine expression and apoptotic cell death, which may be regulated by NF-kappaB, AP-1, and possibly MAPK in pancreatic acinar cells.
Full Text
http://onlinelibrary.wiley.com/doi/10.1196/annals.1299.017/abstract
DOI
OAK-2003-00009
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113169
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