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Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2015-07-15T16:34:25Z | - |
dc.date.available | 2015-07-15T16:34:25Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0077-8923 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113169 | - |
dc.description.abstract | The signaling pathways mediating cytokine production and apoptosis in pancreatic acinar cells have not been fully understood. We investigated the signal transduction of cerulein-induced cytokine IL-6 expression and apoptosis in pancreatic acinar AR42J cells. The wild-type cells and the cells transfected with mutant genes for signaling molecules (such as transcription factor NF-kappaB and AP-1) and ras, the upstream signal for mitogen-activated protein kinase (MAPK), were used. IL-6 level was determined in the medium by enzyme-linked immunosorbent assay (ELISA). Apoptosis was determined by viable cell counts and DNA fragmentation. Wild-type cells and transfected cells with control vector (pcDNA), IkappaB mutant gene (IkappaB mt), H-ras mutant gene (Ras N17), or c-jun dominant negative gene (TAM-67) were treated with cerulein for 24 hours. As a result, cerulein induced IL-6 expression time-dependently at 10(-8) M, and apoptosis dose-dependently at 24 h in the wild-type cells. Cerulein-induced IL-6 expression (at 10(-8) M) and apoptosis (at 10(-7) M) were inhibited in the transfected cells with mutant gene (IkappaB mt, Ras N17, TAM-67) as compared to pcDNA cells and the wild-type cells. In conclusion, cerulein induces cytokine expression and apoptotic cell death, which may be regulated by NF-kappaB, AP-1, and possibly MAPK in pancreatic acinar cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | ANNALS OF THE NEW YORK ACADEMY OF SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Apoptosis/immunology | - |
dc.subject.MESH | Ceruletide/pharmacology* | - |
dc.subject.MESH | Cytokines/genetics* | - |
dc.subject.MESH | Gene ExpressionRegulation/drug effects | - |
dc.subject.MESH | Genes, ras | - |
dc.subject.MESH | Pancreas/cytology* | - |
dc.subject.MESH | Pancreas/drug effects | - |
dc.subject.MESH | Pancreas/physiology | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Signal Transduction/physiology* | - |
dc.subject.MESH | Transfection | - |
dc.title | Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | JANGWON LEE | - |
dc.contributor.googleauthor | JEONGHUN SEO | - |
dc.contributor.googleauthor | KYUNG HWAN KIM | - |
dc.contributor.googleauthor | JAE BOCK CHUNG | - |
dc.contributor.googleauthor | HYEYOUNG KIM | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J00181 | - |
dc.identifier.eissn | 1749-6632 | - |
dc.identifier.pmid | 15033703 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1196/annals.1299.017/abstract | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 1010 | - |
dc.citation.startPage | 104 | - |
dc.citation.endPage | 108 | - |
dc.identifier.bibliographicCitation | ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 104-108, 2003 | - |
dc.identifier.rimsid | 54181 | - |
dc.type.rims | ART | - |
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