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Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells

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dc.contributor.author김경환-
dc.date.accessioned2015-07-15T16:34:25Z-
dc.date.available2015-07-15T16:34:25Z-
dc.date.issued2003-
dc.identifier.issn0077-8923-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113169-
dc.description.abstractThe signaling pathways mediating cytokine production and apoptosis in pancreatic acinar cells have not been fully understood. We investigated the signal transduction of cerulein-induced cytokine IL-6 expression and apoptosis in pancreatic acinar AR42J cells. The wild-type cells and the cells transfected with mutant genes for signaling molecules (such as transcription factor NF-kappaB and AP-1) and ras, the upstream signal for mitogen-activated protein kinase (MAPK), were used. IL-6 level was determined in the medium by enzyme-linked immunosorbent assay (ELISA). Apoptosis was determined by viable cell counts and DNA fragmentation. Wild-type cells and transfected cells with control vector (pcDNA), IkappaB mutant gene (IkappaB mt), H-ras mutant gene (Ras N17), or c-jun dominant negative gene (TAM-67) were treated with cerulein for 24 hours. As a result, cerulein induced IL-6 expression time-dependently at 10(-8) M, and apoptosis dose-dependently at 24 h in the wild-type cells. Cerulein-induced IL-6 expression (at 10(-8) M) and apoptosis (at 10(-7) M) were inhibited in the transfected cells with mutant gene (IkappaB mt, Ras N17, TAM-67) as compared to pcDNA cells and the wild-type cells. In conclusion, cerulein induces cytokine expression and apoptotic cell death, which may be regulated by NF-kappaB, AP-1, and possibly MAPK in pancreatic acinar cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent104~108-
dc.relation.isPartOfANNALS OF THE NEW YORK ACADEMY OF SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHApoptosis/immunology-
dc.subject.MESHCeruletide/pharmacology*-
dc.subject.MESHCytokines/genetics*-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHGenes, ras-
dc.subject.MESHPancreas/cytology*-
dc.subject.MESHPancreas/drug effects-
dc.subject.MESHPancreas/physiology-
dc.subject.MESHRats-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHSignal Transduction/physiology*-
dc.subject.MESHTransfection-
dc.titleSignal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJANGWON LEE-
dc.contributor.googleauthorJEONGHUN SEO-
dc.contributor.googleauthorKYUNG HWAN KIM-
dc.contributor.googleauthorJAE BOCK CHUNG-
dc.contributor.googleauthorHYEYOUNG KIM-
dc.identifier.doiOAK-2003-00009-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00311-
dc.relation.journalcodeJ00181-
dc.identifier.eissn1749-6632-
dc.identifier.pmid15033703-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1196/annals.1299.017/abstract-
dc.subject.keyword15033703-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.rights.accessRightsnot free-
dc.citation.volume1010-
dc.citation.startPage104-
dc.citation.endPage108-
dc.identifier.bibliographicCitationANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1010 : 104-108, 2003-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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