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A stop-EGFP transgenic mouse to detect clonal cell lineages generated by mutation

Authors
 Simon Ro  ;  Bruce Rannala 
Citation
 EMBO JOURNAL, Vol.5(9) : 914-920, 2004 
Journal Title
 EMBO JOURNAL 
ISSN
 0261-4189 
Issue Date
2004
MeSH
Animals ; Epidermis/metabolism* ; Fluorescent Dyes ; Genes, Reporter* ; Indoles ; Kidney/cytology ; Kidney/metabolism ; Liver/cytology ; Liver/metabolism ; Mice ; Mice, Transgenic ; Mutation* ; NIH 3T3 Cells ; Stem Cells/metabolism*
Keywords
clonal cell lineage ; enhanced green fluorescent protein ; epidermal stem cell ; in vivo imaging ; mutation
Abstract
The investigation of cell lineages and clonal organization in tissues is facilitated by techniques that allow labelling of clonal cell lineages. Here, we describe a novel transgenic mouse that allows clonal cell lineages to be traced in virtually any tissue. A green fluorescent cell lineage is generated by a random mutation at an enhanced green fluorescent protein gene that carries a premature stop codon, ensuring clonality. The transgenic system allows efficient detection of mutations and stem-cell fate mapping in the epidermis using live mice, as well as in the kidney and liver post-mortem. Cell lineages that descended from single epidermal stem cells were found to be capable of generating three adjacent corneocytes using the system, providing evidence for horizontal migration of epidermal cells between epidermal proliferative units (EPUs), in contrast to the classical EPU model. The transgenic mouse system is expected to provide a novel tool for stem-cell lineage studies.
Files in This Item:
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DOI
10.1038/sj.embor.7400218
Appears in Collections:
5. Research Institutes (연구소) > Liver Cirrhosis Clinical Research Center (간경변증임상연구센터) > 1. Journal Papers
Yonsei Authors
Ro, Simon Weonsang(노원상) ORCID logo https://orcid.org/0000-0003-2187-3698
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112950
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