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Cited 37 times in

A stop-EGFP transgenic mouse to detect clonal cell lineages generated by mutation

DC Field Value Language
dc.contributor.author노원상-
dc.date.accessioned2015-07-14T17:28:19Z-
dc.date.available2015-07-14T17:28:19Z-
dc.date.issued2004-
dc.identifier.issn0261-4189-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/112950-
dc.description.abstractThe investigation of cell lineages and clonal organization in tissues is facilitated by techniques that allow labelling of clonal cell lineages. Here, we describe a novel transgenic mouse that allows clonal cell lineages to be traced in virtually any tissue. A green fluorescent cell lineage is generated by a random mutation at an enhanced green fluorescent protein gene that carries a premature stop codon, ensuring clonality. The transgenic system allows efficient detection of mutations and stem-cell fate mapping in the epidermis using live mice, as well as in the kidney and liver post-mortem. Cell lineages that descended from single epidermal stem cells were found to be capable of generating three adjacent corneocytes using the system, providing evidence for horizontal migration of epidermal cells between epidermal proliferative units (EPUs), in contrast to the classical EPU model. The transgenic mouse system is expected to provide a novel tool for stem-cell lineage studies.-
dc.description.statementOfResponsibilityopen-
dc.format.extent914~920-
dc.relation.isPartOfEMBO JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHEpidermis/metabolism*-
dc.subject.MESHFluorescent Dyes-
dc.subject.MESHGenes, Reporter*-
dc.subject.MESHIndoles-
dc.subject.MESHKidney/cytology-
dc.subject.MESHKidney/metabolism-
dc.subject.MESHLiver/cytology-
dc.subject.MESHLiver/metabolism-
dc.subject.MESHMice-
dc.subject.MESHMice,Transgenic-
dc.subject.MESHMutation*-
dc.subject.MESHNIH 3T3 Cells-
dc.subject.MESHStem Cells/metabolism*-
dc.titleA stop-EGFP transgenic mouse to detect clonal cell lineages generated by mutation-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentLiver Cirrhosis Clinical Research Center (간경변증임상연구센터)-
dc.contributor.googleauthorSimon Ro-
dc.contributor.googleauthorBruce Rannala-
dc.identifier.doi10.1038/sj.embor.7400218-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01286-
dc.relation.journalcodeJ00763-
dc.identifier.eissn1460-2075-
dc.identifier.pmid15297872-
dc.subject.keywordclonal cell lineage-
dc.subject.keywordenhanced green fluorescent protein-
dc.subject.keywordepidermal stem cell-
dc.subject.keywordin vivo imaging-
dc.subject.keywordmutation-
dc.contributor.alternativeNameRo, Simon Weonsang-
dc.contributor.affiliatedAuthorRo, Simon Weonsang-
dc.rights.accessRightsfree-
dc.citation.volume5-
dc.citation.number9-
dc.citation.startPage914-
dc.citation.endPage920-
dc.identifier.bibliographicCitationEMBO JOURNAL, Vol.5(9) : 914-920, 2004-
dc.identifier.rimsid36818-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers

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