143 338

Cited 0 times in

Modulation of the Surface Expression of CD158 Killer Cell Ig-like Receptor by Interleukin-2 and Transforming Growth Factor-β

 ui Cheol Shin  ;  Kyung Seon Choi  ;  Jeon Soo Shin  ;  Se Jong Kim 
 YONSEI MEDICAL JOURNAL, Vol.45(3) : 510-514, 2004 
Journal Title
Issue Date
NK cells ; killer cell Ig-like receptor ; IL-2 ; TGF-β
Killer cell Ig-like receptor (KIR) binds to HLA class I molecules on the surface of target cells, and it confers inhibitory signals to NK cells. Although NK cytotoxicity can be affected by the change of the surface expression of KIR on NK cells, the effect of cytokines on the regulation of KIR expression has not been thoroughly investigated. Here in our study, we investigated the effect of several cytokines, including IL-2, TGF-β, IFN-γ, IL-12 and IL-18, on the surface expression of CD158 KIR, which binds to HLA-C, by the use of FACS analysis. In the isolated NK cells, IL-2 obviously increased the surface expression of CD158 KIR after 72 hr in vitro culture, and this was evidenced by the increased percentage of CD158+ NK cells and the increased mean fluorescence intensity of CD158 in CD158+ NK cells. In contrast, TGF-β decreased the surface expression of CD158 KIR after 72 hr culture. However, IFN-γ, IL-12 and IL-18 did not change the expression of CD158 KIR. The modulated expression of KIR by IL-2 and TGF-β can be associated with the changed NK-cytotoxic target-discriminating ability of NK cells upon their exposure to IL-2 and TGF-β.
Files in This Item:
T200403562.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Shin, Ui Cheol(신의철)
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.