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Migration of Osteoblast-like Cells on Extracellular Matrices-coated Culture Plate Using Cell Tracking System

 Hyun Joo Son  ;  Min Sub Lee  ;  Jong Chul Park  ;  Hideki Tatsunoto  ;  Motoi Machida  ;  Jong Suk Park  ;  Bong Joo Park  ;  Dong Wook Han 
 Biomaterials Research (생체재료학회지), Vol.8(2) : 120-124, 2004 
Journal Title
 Biomaterials Research (생체재료학회지) 
Issue Date
Cell migration ; Extracellular matrix ; Osteoblast-like cell ; Cell tracking system
In this study, the migration of MC3T3-E1 osteoblast-like cells was examined on the culture plate coated with ECM, such as type I collagen, laminin or fibronectin. The cells were incubated in a self-made mini-incubator, with the same conditions as commercial CO2 incubator, placed on an inverted microscope and observed as a real time-mode. After 24 hr of incubation, the edge and center of single cell were detected by an automated cell tracking system. From the cell movement path, it was shown that the cells had a tendency to move randomly without any certain direction. Furthermore, it could be found that the cell movement speed was divided into three phases, attachment preparation phase, from 0 to T1 hr, accelerated movement phase, from T1 to T2 hr and speed maintenance phase, till 24 hr. At first phase, a cell moved slightly as like searching a local attachment site. Afterwards, the cell accelerated its speed and reached the maximum speed, Vmax. To estimate the cell migration speed, the migration index was defined as the value of the Vmax divided by T2-T1. The migration index of the MC3T3-E1 cells on fibronectin was 8.9, while those indexes on the type I collagen and laminin were respectively 5.3 and 5.1. These results suggested that all cells might have their own migration index according to their species or phenotypes and ECM type, and the migration speed could be inferred from those values without further study.
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Son, Hyun Joo(손현주)
Lee, Min Sub(이민섭)
Lee, Min Young(이민영)
Han, Dong Wook(한동욱)
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