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자외선 조사를 한 수정체 상피세포에서 Nuclear Factor Kappa B의 활성화에 관한 연구

Other Titles
 The Role of Nuclear Factor Kappa B on Lens Epithelial Cells after Ultraviolet B Irradiation 
Authors
 이도형  ;  김정권  ;  오세훈  ;  김응권  ;  주천기 
Citation
 JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY, Vol.45(3) : 500-506, 2004 
Journal Title
 JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY 
ISSN
 0378-6471 
Issue Date
2004
Keywords
Human lens epithelial cells ; Nuclear factor kappa B ; Ultraviolet irradiation
Abstract
Purpose: This study was performed to determine the role of nuclear factor kappa B (NF-κ B) on the lens epithelial cell death after ultraviolet (UV) irradiation. Methods: Simian virus 40 transfected human lens epithelial cells (HLE B-3 cells) were used in this study. UVB located at 10cm from the bottom was irradiated during 1, 2, 3 and 4 minutes. To measure the cytotoxicity N= .assay was used. Translocation of NF-x B was examined by immunocytochemistry with anti NF-κ B p65 antibody and electrophoretic mobility shift assay (EMSA). To confirm the role of NF-κ B, the cells were pretreated with sulfasalazine, a specific inhibitor of NF-κ B, for 30 minutes before irradiation, and cytotoxicity and translocation of NF-κ B were evaluated. . Results: UV irradiation produced a progressive cytotoxic effect in cultured HLE B-3 cells after 1 minute and maximum cytotoxicity was reached after 3 minutes irradiation. When HLE B-3 cells were irradia´ted with UVB, the translocation of NF-κ B was observed in immunocytochemistry. These translocations were peaked 6 hours after UV irradiation in EMSA. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-κ B was blocked. The cellular death after UV irradiation was markedly blocked by sulfasalazine. UV irradiation can translocate NF-κ B and sulfasalazine is a useful blocking agent in this pathway. In addition, sulfasalazine can prevent cellular death after UV irradiation. Conclusions: These findings suggest that NF-κ B plays an important role in cellular death after UV irradiation.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112234
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