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자외선 조사를 한 수정체 상피세포에서 Nuclear Factor Kappa B의 활성화에 관한 연구
DC Field | Value | Language |
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dc.contributor.author | 김응권 | - |
dc.date.accessioned | 2015-07-14T17:06:51Z | - |
dc.date.available | 2015-07-14T17:06:51Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0378-6471 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112234 | - |
dc.description.abstract | Purpose: This study was performed to determine the role of nuclear factor kappa B (NF-κ B) on the lens epithelial cell death after ultraviolet (UV) irradiation. Methods: Simian virus 40 transfected human lens epithelial cells (HLE B-3 cells) were used in this study. UVB located at 10cm from the bottom was irradiated during 1, 2, 3 and 4 minutes. To measure the cytotoxicity N= .assay was used. Translocation of NF-x B was examined by immunocytochemistry with anti NF-κ B p65 antibody and electrophoretic mobility shift assay (EMSA). To confirm the role of NF-κ B, the cells were pretreated with sulfasalazine, a specific inhibitor of NF-κ B, for 30 minutes before irradiation, and cytotoxicity and translocation of NF-κ B were evaluated. . Results: UV irradiation produced a progressive cytotoxic effect in cultured HLE B-3 cells after 1 minute and maximum cytotoxicity was reached after 3 minutes irradiation. When HLE B-3 cells were irradia´ted with UVB, the translocation of NF-κ B was observed in immunocytochemistry. These translocations were peaked 6 hours after UV irradiation in EMSA. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-κ B was blocked. The cellular death after UV irradiation was markedly blocked by sulfasalazine. UV irradiation can translocate NF-κ B and sulfasalazine is a useful blocking agent in this pathway. In addition, sulfasalazine can prevent cellular death after UV irradiation. Conclusions: These findings suggest that NF-κ B plays an important role in cellular death after UV irradiation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 500~506 | - |
dc.relation.isPartOf | JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 자외선 조사를 한 수정체 상피세포에서 Nuclear Factor Kappa B의 활성화에 관한 연구 | - |
dc.title.alternative | The Role of Nuclear Factor Kappa B on Lens Epithelial Cells after Ultraviolet B Irradiation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | 이도형 | - |
dc.contributor.googleauthor | 김정권 | - |
dc.contributor.googleauthor | 오세훈 | - |
dc.contributor.googleauthor | 김응권 | - |
dc.contributor.googleauthor | 주천기 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J01838 | - |
dc.identifier.eissn | 2092-9374 | - |
dc.subject.keyword | Human lens epithelial cells | - |
dc.subject.keyword | Nuclear factor kappa B | - |
dc.subject.keyword | Ultraviolet irradiation | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 45 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 500 | - |
dc.citation.endPage | 506 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY, Vol.45(3) : 500-506, 2004 | - |
dc.identifier.rimsid | 37988 | - |
dc.type.rims | ART | - |
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