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자외선 조사를 한 수정체 상피세포에서 Nuclear Factor Kappa B의 활성화에 관한 연구

DC FieldValueLanguage
dc.contributor.author김응권-
dc.date.accessioned2015-07-14T17:06:51Z-
dc.date.available2015-07-14T17:06:51Z-
dc.date.issued2004-
dc.identifier.issn0378-6471-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/112234-
dc.description.abstractPurpose: This study was performed to determine the role of nuclear factor kappa B (NF-κ B) on the lens epithelial cell death after ultraviolet (UV) irradiation. Methods: Simian virus 40 transfected human lens epithelial cells (HLE B-3 cells) were used in this study. UVB located at 10cm from the bottom was irradiated during 1, 2, 3 and 4 minutes. To measure the cytotoxicity N= .assay was used. Translocation of NF-x B was examined by immunocytochemistry with anti NF-κ B p65 antibody and electrophoretic mobility shift assay (EMSA). To confirm the role of NF-κ B, the cells were pretreated with sulfasalazine, a specific inhibitor of NF-κ B, for 30 minutes before irradiation, and cytotoxicity and translocation of NF-κ B were evaluated. . Results: UV irradiation produced a progressive cytotoxic effect in cultured HLE B-3 cells after 1 minute and maximum cytotoxicity was reached after 3 minutes irradiation. When HLE B-3 cells were irradia´ted with UVB, the translocation of NF-κ B was observed in immunocytochemistry. These translocations were peaked 6 hours after UV irradiation in EMSA. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-κ B was blocked. The cellular death after UV irradiation was markedly blocked by sulfasalazine. UV irradiation can translocate NF-κ B and sulfasalazine is a useful blocking agent in this pathway. In addition, sulfasalazine can prevent cellular death after UV irradiation. Conclusions: These findings suggest that NF-κ B plays an important role in cellular death after UV irradiation.-
dc.description.statementOfResponsibilityopen-
dc.format.extent500~506-
dc.relation.isPartOfJOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title자외선 조사를 한 수정체 상피세포에서 Nuclear Factor Kappa B의 활성화에 관한 연구-
dc.title.alternativeThe Role of Nuclear Factor Kappa B on Lens Epithelial Cells after Ultraviolet B Irradiation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthor이도형-
dc.contributor.googleauthor김정권-
dc.contributor.googleauthor오세훈-
dc.contributor.googleauthor김응권-
dc.contributor.googleauthor주천기-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ01838-
dc.identifier.eissn2092-9374-
dc.subject.keywordHuman lens epithelial cells-
dc.subject.keywordNuclear factor kappa B-
dc.subject.keywordUltraviolet irradiation-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.rights.accessRightsfree-
dc.citation.volume45-
dc.citation.number3-
dc.citation.startPage500-
dc.citation.endPage506-
dc.identifier.bibliographicCitationJOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY, Vol.45(3) : 500-506, 2004-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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