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당뇨병 환자에서 산화 스트레스에 의한 NF-κB 및 AP-1의 활성화가 죽상동맥경화증에 미치는 영향

Other Titles
 Role of Activation of NF- B and AP-1 by Oxidative Stress in Atherosclerosis in Diabetic Patients 
Authors
 김철식  ;  이근택  ;  이현철  ;  김경래  ;  임승길  ;  차봉수  ;  안철우  ;  김똘미  ;  박종숙  ;  남주영  ;  조민호  ;  박진아 
Citation
 Journal of Korean Diabetes Association (당뇨병), Vol.28(4) : 255-264, 2004 
Journal Title
 Journal of Korean Diabetes Association  (당뇨병) 
ISSN
 1976-9180 
Issue Date
2004
Abstract
Background: The aim of this study was to evaluate the possible role of NF- B activation and AP-1 by oxidative stress in atherosclerosis in diabetic patients by measuring the carotid intima-media thickness, intracellular ROS generation and activation of transcription factors, including nuclear factor-kappa B (NF- B) and activator protein-1 (AP-1). Methods: Sixty-six patients (28 males, 38 females; age 56.1 13.4 years; duration of diabetes 115.7 83.4 months) with type 2 diabetes mellitus (DM) were selected for this study. The DM patients included in this study were divided into those with a normal carotid intima-media thickness (Group II) and those with an increased intima-media thickness (Group III). 57 healthy controls matched for age and sex with the DM patients (Group I) were randomly selected. Dichlorodifluorescein (DCF)-sensitive intracellular ROS was measured by fluorescent spectrometry. The activities of NF- B and AP-1 in PBMCs were measured by an electrophoretic mobility shift assay. Results: No differences were evident between the groups in terms of gender, age, BMI, blood pressure, total cholesterol, triglyceride, LDL-cholesterol and HDL-cholesterol. Spontaneous and H2O2 (or phorbol-12-myristate-13-acetate, PMA) stimulated ROS were significantly higher in the PBMCs from the DM patients with an increased intima-media thickness (Group III) than in those without (Group II), and were also higher in the control group (Group I). Moreover, the activities of NF- B and AP-1 were significantly higher in Group III than in Groups I or II. Conclusion: The present study demonstrates that intracellular ROS generation, and NF- B and AP-1 activation in PBMCs strongly correlates with the carotid artery IMT. These clinical results suggest that increased oxidative stress in PBMCs may play a role in the pathogenesis of atherosclerosis in DM patients
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
김경래(Kim, Kyung Rae)
김똘미(Kim, Dol Mi)
김철식(Kim, Chul Sik)
남주영(Nam, Ju Young)
박종숙(Park, Jong Suk) ORCID logo https://orcid.org/0000-0002-5385-1373
박진아(Park, Jina)
안철우(Ahn, Chul Woo)
이현철(Lee, Hyun Chul)
임승길(Lim, Sung Kil)
차봉수(Cha, Bong Soo) ORCID logo https://orcid.org/0000-0003-0542-2854
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111906
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