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Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas

 Jun Haeng Lee  ;  Hyung Keun Lee  ;  SoonWon Hong  ;  Gong Je Seong  ;  Eung Kweon Kim  ;  Sung Eun Kim  ;  Jee Ho Chang  ;  Jin Kuk Kim 
 Journal of Cataract and Refractive Surgery, Vol.30(10) : 2192-2199, 2004 
Journal Title
 Journal of Cataract and Refractive Surgery 
Issue Date
PURPOSE: To investigate the expression of laminin-5 during epithelial healing and evaluate its expression in vivo using rat corneas on which amniotic membrane was applied to cover the wound after excimer laser photoablation. SETTING: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. METHODS: Myopic photorefractive keratectomy (PRK) with a 100 microm deep ablation was performed in Sprague Dawley rats killed 6, 12, 24, 48, 72, and 96 hours after the procedure. In the first group of 30 rats, the excimer laser-ablated cornea was covered with amniotic membrane after PRK. Thirty other rats in which no amniotic membrane treatment was used served as controls. Immunohistochemical and immunofluoresce in techniques were used to monitor the expression of laminin-5, gamma2, and gamma1 in the rat corneas. Immunoblotting was used to compare the expression of laminin between the amniotic membrane group and the control group. RESULTS: In the immunoblotting study, laminin-5, alpha3, and gamma2 increased 24 hours after amniotic membrane treatment compared to the control group. At 12 hours, in vivo immunostaining of the corneas in both groups expressed laminin, but laminin-5 and gamma2 were more intensely expressed in the amniotic membrane group. This continued until reepithelialization. Expression of the gamma1 chain was not different between the 2 groups. CONCLUSION: With the use of amniotic membrane, the expression of laminin-5 and gamma2 was faster and more intense than in a control group during reepithelialization of excimer laser-ablated rat corneas.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Eun(김성은)
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
Lee, Joon Haeng(이준행)
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
Chang, Jee Ho(장지호)
Hong, Soon Won(홍순원) ORCID logo https://orcid.org/0000-0002-0324-2414
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