1 372

Cited 3 times in

Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas

DC FieldValueLanguage
dc.contributor.author김성은-
dc.contributor.author김응권-
dc.contributor.author성공제-
dc.contributor.author이준행-
dc.contributor.author이형근-
dc.contributor.author장지호-
dc.contributor.author홍순원-
dc.date.accessioned2015-07-14T16:49:47Z-
dc.date.available2015-07-14T16:49:47Z-
dc.date.issued2004-
dc.identifier.issn0886-3350-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111673-
dc.description.abstractPURPOSE: To investigate the expression of laminin-5 during epithelial healing and evaluate its expression in vivo using rat corneas on which amniotic membrane was applied to cover the wound after excimer laser photoablation. SETTING: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. METHODS: Myopic photorefractive keratectomy (PRK) with a 100 microm deep ablation was performed in Sprague Dawley rats killed 6, 12, 24, 48, 72, and 96 hours after the procedure. In the first group of 30 rats, the excimer laser-ablated cornea was covered with amniotic membrane after PRK. Thirty other rats in which no amniotic membrane treatment was used served as controls. Immunohistochemical and immunofluoresce in techniques were used to monitor the expression of laminin-5, gamma2, and gamma1 in the rat corneas. Immunoblotting was used to compare the expression of laminin between the amniotic membrane group and the control group. RESULTS: In the immunoblotting study, laminin-5, alpha3, and gamma2 increased 24 hours after amniotic membrane treatment compared to the control group. At 12 hours, in vivo immunostaining of the corneas in both groups expressed laminin, but laminin-5 and gamma2 were more intensely expressed in the amniotic membrane group. This continued until reepithelialization. Expression of the gamma1 chain was not different between the 2 groups. CONCLUSION: With the use of amniotic membrane, the expression of laminin-5 and gamma2 was faster and more intense than in a control group during reepithelialization of excimer laser-ablated rat corneas.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2192~2199-
dc.relation.isPartOfJOURNAL OF CATARACT AND REFRACTIVE SURGERY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleExpression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorJun Haeng Lee-
dc.contributor.googleauthorHyung Keun Lee-
dc.contributor.googleauthorSoonWon Hong-
dc.contributor.googleauthorGong Je Seong-
dc.contributor.googleauthorEung Kweon Kim-
dc.contributor.googleauthorSung Eun Kim-
dc.contributor.googleauthorJee Ho Chang-
dc.contributor.googleauthorJin Kuk Kim-
dc.identifier.doi10.1016/j.jcrs.2004.02.021-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ01299-
dc.identifier.eissn1873-4502-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0886335004001658-
dc.contributor.alternativeNameKim, Sung Eun-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.contributor.alternativeNameSeong, Gong Je-
dc.contributor.alternativeNameLee, Joon Haeng-
dc.contributor.alternativeNameLee, Hyung Keun-
dc.contributor.alternativeNameChang, Jee Ho-
dc.contributor.alternativeNameHong, Soon Won-
dc.rights.accessRightsnot free-
dc.citation.volume30-
dc.citation.number10-
dc.citation.startPage2192-
dc.citation.endPage2199-
dc.identifier.bibliographicCitationJOURNAL OF CATARACT AND REFRACTIVE SURGERY, Vol.30(10) : 2192-2199, 2004-
dc.identifier.rimsid37404-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.