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Effects of green tea polyphenol on preservation of human saphenous vein

 Dong-Wook Han  ;  Young Hwan Park  ;  Jong-Chul Park  ;  Hwal Suh  ;  Suong-Hyu Hyon  ;  Kwon-Yong Lee  ;  Jeong Koo Kim 
 JOURNAL OF BIOTECHNOLOGY, Vol.110(2) : 109-117, 2004 
Journal Title
Issue Date
Blotting, Western ; Cell Survival/drug effects ; Endothelial Cells/drug effects ; Flavonoids/pharmacology* ; Flow Cytometry ; Humans ; In Vitro Techniques ; Nitric Oxide Synthase/biosynthesis ; Nitric Oxide Synthase Type III ; Phenols/pharmacology* ; Plant Extracts/pharmacology ; Polyphenols ; Saphenous Vein/cytology ; Saphenous Vein/drug effects* ; Tea ; Tissue Preservation/methods*
Green tea polyphenol ; Human saphenous vein ; Preservation ; Cell viability ; Endothelial nitric oxide synthase
The potential role of green tea polyphenol (GtPP) in preserving the human saphenous vein was investigated under physiological conditions. The vein segments were incubated for 1, 3, 5, 7 and 14 days, either after 4h of treatment with 1.0mg/ml GtPP or in the presence of GtPP at the same concentration. After incubation, the endothelial cell viability, endothelial nitric oxide synthase (eNOS) expression and the vein histology were evaluated. When the veins were not treated with GtPP, the viability of the endothelial cells was significantly reduced with the progress in the culture time, and none of the cells expressed eNOS after 5 days. Furthermore, severe histological changes and structural damage were observed in the non-treated veins. In contrast, incubating the veins after 4h of GtPP treatment significantly prevented these phenomena. The cellular viability of the GtPP-treated vein was approximately 64% after 7 days, and eNOS expression was maintained up to 40%, compared to that of the fresh vein. The histological observations showed that the vasculature was quite similar to that of the fresh vein. When incubated with GtPP, the vein could also be preserved for 1 week under physiological conditions retaining both its cellular viability (61%) and eNOS expression level (45%) and maintaining its venous structure without any morphological changes. These results demonstrate that GtPP treatment may be a useful method for preserving the HSV.
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1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
Han, Dong Wook(한동욱)
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