2 356

Cited 244 times in

Cryopyrin and pyrin activate caspase-1, but not NF-kappaB, via ASC oligomerization

Authors
 J-W Yu  ;  J Wu  ;  Z Zhang  ;  P Datta  ;  I Ibrahimi  ;  S Taniguchi  ;  J Sagara  ;  T Fernandes-Alnemri  ;  E S Alnemri 
Citation
 CELL DEATH AND DIFFERENTIATION, Vol.13(2) : 236-249, 2006 
Journal Title
CELL DEATH AND DIFFERENTIATION
ISSN
 1350-9047 
Issue Date
2006
Keywords
inflammation ; inflammasome ; pyrin ; cryopyrin ; caspase-1 ; ASC ; interleukin-1b ; NF-kB
Abstract
Mutations in cryopyrin and pyrin proteins are responsible for several autoinflammatory disorders in humans, suggesting that these proteins play important roles in regulating inflammation. Using a HEK293 cell-based reconstitution system that stably expresses ASC and procaspase-1 we demonstrated that neither cryopyrin nor pyrin or their corresponding disease-associated mutants could significantly activate NF-kappaB in this system. However, both cryopyrin and two disease-associated cryopyrin mutants induced ASC oligomerization and ASC-dependent caspase-1 activation, with the disease-associated mutants being more potent than the wild-type (WT) cryopyrin, because of increased self-oligomerization. Contrary to the proposed anti-inflammatory activity of WT pyrin, our results demonstrated that pyrin, like cryopyrin, can also assemble an inflammasome complex with ASC and procaspase-1 leading to ASC oligomerization, caspase-1 activation and interleukin-1beta processing. Thus, we propose that pyrin could function as a proinflammatory molecule.
Full Text
http://www.nature.com/cdd/journal/v13/n2/abs/4401734a.html
DOI
10.1038/sj.cdd.4401734
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yu, Je Wook(유제욱) ORCID logo https://orcid.org/0000-0001-5943-4071
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111113
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links