조직 재생을 위한 Poly (D, L-lactide-co-glycolide) 표면에 (1→3)(1→6)-ßglucan 고정에 대한 세포 점착 및 성장 효과

Abstract

We examined the effects of -glucan-reinforced PLGA film and scaffold on HDFs (human dermal fibroblast) attachment and proliferation. The PLGA films were prepared by simple solvent-casting method. The prepared films were grafted with (1→3)(1→6)-ß-glucan in various ratios after plasma treatment on surface. The surface of the film was characterized by contact angle measure ment, scanning electron microscope (SEM), and Fourier-transform infrared spectrophotometer (FT-IR). The amount of (1→3)(1→6)-ß-glucan in the prepared film was indirectly determined by phenol-sulfuric acid method. The HDFs (Human dermal fibroblasts) were used to evaluate the cell attachment and proliferation on PLGA specimens before and after plasma/ß-glucan treatment. The result showed that the plasma treated groups exhibited more mont of ß-glucan might be grafted than the non plasma treated groups. Cell attachment was significantly enhanced in the plasma/ß-glucan grafted group after 4 hours incubation (p<0.05) due to the improved hydrophilicity and cytoactivity effect of the ß-glucan The cell proliferation of plasma/ß-glucan(2mg/ml) grafted group was the highest rate among the groups (p<0.05).